現京都大学研究者の神戸理研CDB時代の若山研での2008年論文2本のタイトル
Generation of mice derived from embryonic stem cells using blastocysts different developmental ages
もう一本は
Increasing the Cell Nunber of Host Tetraploid Embryos Can Improve the Production of mice Derived from Embryonic stem Cells
です。確認下さいませ。
2005年論文もあります。
2018/4/8(日) 午前 3:08[ Ooboe ]返信する
上記、アーティクル論文の対応個所は以下である。
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We tested the following three different genetic backgrounds of mice for STAP stem-cell establishment from STAP cell clusters, and observed reproducible data of establishment: C57BL/6 carrying Oct4-gfp (29 of 29), 129/Sv carrying Rosa26-gfp (2 of 2) and 129/Sv × C57BL/6 carrying cag-gfp (12 of 16). STAP stem cells with all these genetic backgrounds showed chimaera-forming activity.
また、この小保方さんはRosa26-gfp に関してはLetter Figure 1-aに関する以下の本文上で129/Sv carrying Rosa26-gfp背景のESがあるという認識でいる。
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We became particularly interested in this question after a blastocyst injection assay revealed an unexpected finding. In general, progeny of injected ES cells are found in the embryonic portion of the chimaera, but rarely in the placental portion (Fig. 1a; shown with Rosa26-GFP). Surprisingly, injected STAP cells contributed not only to the embryo but also to the placenta and fetal membranes (Fig. 1b and Extended Data Fig. 1a–c) in 60% of the chimaeric embryos (Fig. 1c).