そういうことだね。これらの結果はアーティクルのExtended Data Figure7のbにある。
これは数からして一回の実験くさいかな。後のGLSを作った時のキメラ実験の結果も
加えられているようには思いにくいね。一つにはもう10daysはやらないだろうからね。
まして<未知の（16 of 16）>の時のキメラ実験の結果が加わっていたらこんな数ではないと
思われるよ。するとこの表は2011年の実験臭いよね。そこにB6GFPxDBA/2があるんだよね。
しかも罫線でそれだけがポツンと区分けされている。
小保方さんはSTAP細胞について言っていて、STAP幹細胞からのキメラの
ジャームライン実験について言ってるのではないわね。つまりこの表の
実験に関して言っている。これは行っていることはインブリードだわね。
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Because the number of CD45+ cells from a neonatal spleen was small, we mixed spleen cells from male and female mice for STAP cell conversion. To make germline transmission more efficient, we intercrossed chimaeras in some experiments.
その通りだ。ホームズ。小保方さんは2Nキメラの交配実験に関して
To make germline transmission more efficient, we intercrossed chimaeras in some experiments.
と書いた。つまり、この５例のメイティングに関してインタークロスでない実験も行ったという意味になる。
はい。
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Chimaeric mouse generation and analyses
For production of diploid and tetraploid chimaeras with STAP cells, diploid embryos were obtained from ICR strain females. Tetraploid embryos were produced by electrofusion of 2-cell embryos. Because trypsin treatment of donor samples turned out to cause low chimaerism, STAP spherical colonies were cut into small pieces using a microknife under the microscope, and small clusters of STAP cells were then injected into day-4.5 blastocysts by a large pipette. The next day, the chimaeric blastocysts were transferred into day-2.5 pseudopregnant females. For experiments using STAP cells from CD45+ cells without the Oct4-gfp reporter, STAP cell clusters were identified by their characteristic cluster morphology (they are made of very small cells with no strong compaction in the aggregate). When the STAP conversion conditions (low pH) were applied to CD45+ lymphocytes, most day-7 clusters that were large and contained more than a few dozen small cells were positive for Oct4 (although the expression level varied).
（続き）
Therefore, we used only well-formed characteristic clusters (large ones) for this type of study and cut them by microknife to prepare donor cell clusters in a proper size for glass needle injection. For an estimate of the contribution of these injected cells, we used STAP cells that were generated from CD45+ cells of mice constitutively expressing GFP (C57BL/6 line with cag-gfp transgenes; F1 of C57BL/6 and 129/Sv or DBA/2 was used from the viewpoint of heterosis).
Because the number of CD45+ cells from a neonatal spleen was small, we mixed spleen cells from male and female mice for STAP cell conversion. To make germline transmission more efficient, we intercrossed chimaeras in some experiments.
（続き）
For the production of diploid and tetraploid chimaeras with STAP stem cells, diploid embryos were obtained from ICR strain females. Tetraploid embryos were produced by electrofusion of 2-cell embryos. STAP stem cells were dissociated into single cells and injected into day-4.5 blastocysts. In the chimaera studies with both STAP cells and STAP stem cells, we did not find tumorigenetic tendencies in their chimaeras or their offspring (up to 18 months).
翻訳機さんがちゃんと訳してくれてるからわかったわよ。
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In the chimaera studies with both STAP cells and STAP stem cells, we did not find tumorigenetic tendencies in their chimaeras or their offspring (up to 18 months).
STAP細胞とSTAP幹細胞のキメラ研ともに、キメラ或いはその子孫（最大18ヶ月）において腫瘍形成傾向を見出さなかった。
FLSは2012/2/2が最も遅い樹立開始だから、生まれるのは2012/2/22だね。ここから
50日後が2012/4/12で、ここで子供を産ませて20日後の出産が2012/5/1ね。その１８か月後って
2012/10/31だね。
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In the chimaera studies with both STAP cells and STAP stem cells, we did not find tumorigenetic tendencies in their chimaeras or their offspring (up to 18 months).
小町さん、違うわ。それは幹細胞の樹立成績であって、キメラの樹立数とは別よ。
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We tested the following three different genetic backgrounds of mice for STAP stem-cell establishment from STAP cell clusters, and observed reproducible data of establishment: C57BL/6 carrying Oct4-gfp (29 of 29), 129/Sv carrying Rosa26-gfp (2 of 2) and 129/Sv × C57BL/6 carrying cag-gfp (12 of 16). STAP stem cells with all these genetic backgrounds showed chimaera-forming activity.
我々はSTAP細胞塊からSTAP幹細胞を樹立するために以下の3つの異なる遺伝的背景のマウスを試した結果、再現性のある樹立データを観察した：Oct4-gfpの挿入されたC57BL / 6（29の29）、Rosa26-gfpの挿入された129/Sv (2の2）およびcag-gfpの挿入された129 / Sv×C57BL / 6（16の12）。 これらの全ての遺伝的背景を有するSTAP幹細胞はキメラ形成能を示した。
