BCA報告では1から6まで全部解析されたことになってるのよね。その中で
全解析を受けたのは小保方さんが由来不明と証言した理研側の6だわよね。でも
AC129の捏造に使われたのは6番より61番だと結論している。1番は理研側の
保全サンプルには無い。若山さんが持っていたものを解析したのかとも問うて
欲しいわよね。そして、なぜ2013年3月に持ち出した時に理研側に1を残さなかったのかとも。
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☆The genome of 129B6F1 ES6 was sequenced, but further analysis showed that 129B6F1 ES1 rather than 129B6F1 ES6 shares all genomic anomalies found in AC129-1 (see main text).
論文はこれだな。
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Increasing the Cell Number of Host Tetraploid Embryos Can Improve the Production of Mice Derived from Embryonic Stem Cells
Hiroshi Ohta Yuko Sakaide Kazuo Yamagata Teruhiko Wakayama
Biology of Reproduction, Volume 79, Issue 3, 1 September 2008, Pages 486–492,
Published: 01 September 2008
Abstract
Tetraploid (4n) embryo complementation assay has shown that embryonic stem (ES) cells alone are capable of supporting embryonic development (ES mouse), allowing the recovery of mouse lines directly from cultured ES cell lines. Although the advantages of this technique are well recognized, it remains inefficient for generating ES mice. In the present study, we investigated the effects of cell number of host 4n embryos on the production of ES mice. Four independent ES cell lines (two general ES cell lines and two nuclear transfer-derived ES cell lines) were used, and each cell line was aggregated with single (1×) to triple (3×) host 4n embryos. We found that birth rate of ES mice using 1× 4n embryos was quite low (0–2%) regardless of cell line, whereas except for one cell line, approximately 6–14% of embryos developed to full term in the case of 3× 4n embryos. Contamination of host 4n cells in ES mice was quite rare, being comparable to that generated using general methods even if they were delivered from 3× 4n host embryos. These results demonstrate that the use of 3× 4n embryos is effective for generating ES mice. Our technique described here will be applicable to any ES cell line, including general ES cell lines used for gene targeting.
