<③STAP cluster(non-dissociated) 8/20(40%)>は小保方さんとしては
当然GOFマウスで作っているつもりのはずだね。
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d, Teratoma-forming ability of Oct4-GFP+ and Oct4-GFP-dim cells (isolated by FACS, top). Oct4-GFP+ cells, but not Oct4-GFP-dim cells, efficiently formed teratomas (table at the bottom). However, because STAP cells were dissociation-intolerant, the teratoma-forming efficiency of dissociated Oct4-GFP+ cells was lower than that of non-dissociated STAP cell clusters.
小保方さんはこの時渡されていたマウスを129/Sv carrying Rosa26-gfp (2 of 2) だと
思い込まされていたみたいだよね。
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We tested the following three different genetic backgrounds of mice for STAP stem-cell establishment from STAP cell clusters, and observed reproducible data of establishment: C57BL/6 carrying Oct4-gfp (29 of 29), 129/Sv carrying Rosa26-gfp (2 of 2) and 129/Sv × C57BL/6 carrying cag-gfp (12 of 16). STAP stem cells with all these genetic backgrounds showed chimaera-forming activity.
その前の文章から続けて読めばわかるけど、これはSTAP細胞由来マウスの
ジャームライントランスミッション確認実験だ。
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Because the number of CD45+ cells from a neonatal spleen was small, we mixed spleen cells from male and female mice for STAP cell conversion. To make germline transmission more efficient, we intercrossed chimaeras in some experiments.
C57BL/6 carrying Oct4-gfp (29 of 29)はGLSとGLの事ではないよ。驚かないかい?
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We tested the following three different genetic backgrounds of mice for STAP stem-cell establishment from STAP cell clusters, and observed reproducible data of establishment: C57BL/6 carrying Oct4-gfp (29 of 29), 129/Sv carrying Rosa26-gfp (2 of 2) and 129/Sv × C57BL/6 carrying cag-gfp (12 of 16). STAP stem cells with all these genetic backgrounds showed chimaera-forming activity.
キメラが作られているんだよ。
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We tested the following three different genetic backgrounds of mice for STAP stem-cell establishment from STAP cell clusters, and observed reproducible data of establishment: C57BL/6 carrying Oct4-gfp (29 of 29), 129/Sv carrying Rosa26-gfp (2 of 2) and 129/Sv × C57BL/6 carrying cag-gfp (12 of 16). STAP stem cells with all these genetic backgrounds showed chimaera-forming activity.
早く始めとくれ。
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We tested the following three different genetic backgrounds of mice for STAP stem-cell establishment from STAP cell clusters, and observed reproducible data of establishment: C57BL/6 carrying Oct4-gfp (29 of 29), 129/Sv carrying Rosa26-gfp (2 of 2) and 129/Sv × C57BL/6 carrying cag-gfp (12 of 16). STAP stem cells with all these genetic backgrounds showed chimaera-forming activity.
はい、ここからよ。
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We tested the following three different genetic backgrounds of mice for STAP stem-cell establishment from STAP cell clusters, and observed reproducible data of establishment: C57BL/6 carrying Oct4-gfp (29 of 29), 129/Sv carrying Rosa26-gfp (2 of 2) and 129/Sv × C57BL/6 carrying cag-gfp (12 of 16). STAP stem cells with all these genetic backgrounds showed chimaera-forming activity.
我々はSTAP細胞塊からSTAP幹細胞を樹立するために以下の3つの異なる遺伝的背景のマウスを試した結果、再現性のある樹立データを観察した：Oct4-gfpの挿入されたC57BL / 6（29の29）、Rosa26-gfpの挿入された129/Sv (2の2）およびcag-gfpの挿入された129 / Sv×C57BL / 6（16の12）。 これらの全ての遺伝的背景を有するSTAP幹細胞はキメラ形成能を示した。
<①C57BL/6 carrying Oct4-gfp (29 of 29)>は失敗無しの樹立だから<2.GL(8 of 10)>は
カウントされていなくて、もう一つ別に(16 of 16)の実験があったか、もしくは小保方さんは
あったと聞いているということになるのね。そうでないと(29 of 29)にならないとということね。
