そうね。だから何か分かりにくい文章になってるのね。こっちを見た方が早いでしょ。
相澤報告のマテメソだわ。
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Materials and methods
Animals
C57BL/6NJcl and 129X1/SvJJmsSlc mice were purchased from CLEA Japan and Japan SLC, respectively. A transgenic mouse line harboring GFP under an Oct4 promoter (GOF-Tg; Ohbo et al., 2003) was provided by RIKEN BioResource Center (BRC) to CDB, and has been maintained in homozygous state under C57BL/6 background in CDB animal facility. A transgenic mouse line harboring GFP under a CAG promoter (CAG-GFP Tg; Okabe et al., 1997) was provided to CDB by Masaru Okabe at Osaka University, and has been maintained in homozygous state under C57BL/6 background in CDB animal facility. Animals were housed in environmentally controlled rooms, and animal experiments were conducted under the institutional guidelines for Animal and Recombinant DNA Experiments that are consistent with ARRIVE guidelines. The experiments were approved by Institutional Animal Care and Use Committee of RIKEN Kobe Branch (Permit No., AH26-01).
ここでしょ。
>>Chimera production was performed with spleens of a transgenic mouse line harboring GFP under a CAG promoter (Okabe et al., 1997) which were also maintained homozygously in C57BL/6 background; this line is different from the one previously used (Obokata et al., 2014a; Obokata et al., 2014b).
そういう風にレター論文を書いたのは笹井さんで、若山さんは別様に書くつもりであったはずだね。
アーティクルのアブストの出だしは以下だ。
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Here we report a unique cellular reprogramming phenomenon, called stimulus-triggered acquisition of pluripotency (STAP), which requires neither nuclear transfer nor the introduction of transcription factors.
レターも以下だ。
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We recently discovered an unexpected phenomenon of somatic cell reprogramming into pluripotent cells by exposure to sublethal stimuli, which we call stimulus-triggered acquisition of pluripotency (STAP). This reprogramming does not require nuclear transfer or genetic manipulation.
アーティクルのTCRの件は以下ね。
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TCR-β chain gene rearrangement analysis
Genomic DNA was extracted from STAP cells and tail tips from chimaeric mice generated with STAP cells derived from CD45+ cells. PCR was performed with 50 ng DNA using the following primers (Dβ2: 5′-GCACCTGTGGGGAAGAAACT-3′ and Jβ2.6: 5′-TGAGAGCTGTCTCCTACTATCGATT-3′) that amplify the regions of the (D)J recombination. The PCR products were subjected to gel electrophoresis in Tris-acetate-EDTA buffer with 1.6% agarose and visualized by staining with ethidium bromide. PCR bands from STAP cells were subjected to sequencing analysis and identified as rearranged genomic fragments of the (D)J recombination.
我々は小保方さんは無実という立場だからね。当然だけどB6-CAG-GFPx129/Svだと
若山さんに言われたからそう書いていて、かつ親の雌雄の違いは見た目では分からないよね。
そして「あるとき」ではなくて2012年の3月に光る胎盤を伴うキメラ胎児を
受け取って、それを若山さんの指示通りに、切片の免染確認でGFPを胎盤に検出しているのが
Letter Extended Data Figure 1 Placental contribution of STAP cellsなんだよな。
