The D3 ES cell line was originally established by Doetschman et al. [12], and its GFP transgenic cell line (129SV(D3)-Tg(ACTB-EGFP)CZ-001-FM260Osb), which was established using pCAG-EGFP [10, 11], was kindly provided by Dr. Masaru Okabe (Osaka University, Osaka, Japan). The E14 ES cell line [7] was derived from the inbred mouse strain 129/Ola in 1985 by Dr. Martin Hooper in Edinburgh (Scotland) and was obtained through Dr. Peter Mombaerts (Rockefeller University). The 129B6F1G1 [13] and BDmt2 [14] are nuclear transfer-derived ES (ntES) cell lines [15] previously established in our laboratory using Sertoli cells of 129B6F1 background with GFP and tail-tip cells of a male BDF1 mouse as donor for nuclear transfer, respectively. Male 129B6F1 mice expressing GFP were generated by mating female 129/Sv mice with male C57BL/6 GFP transgenic mice (double-transgenic mouse line [16] constructed using pCAG-EGFP [10, 11] and Acr3-EGFP [17]).
あれ、デラ、僕の名前で書きこんでるぜ。
問題は４行目意以降だね。
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he 129B6F1G1 [13] and BDmt2 [14] are nuclear transfer-derived ES (ntES) cell lines [15] previously established in our laboratory using Sertoli cells of 129B6F1 background with GFP and tail-tip cells of a male BDF1 mouse as donor for nuclear transfer, respectively. Male 129B6F1 mice expressing GFP were generated by mating female 129/Sv mice with male C57BL/6 GFP transgenic mice (double-transgenic mouse line [16] constructed using pCAG-EGFP [10, 11] and Acr3-EGFP [17]).
今はBDmt2は関係ないから、その記述を外せばわかりやすいね。
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The 129B6F1G1 [13] ・・・ are nuclear transfer-derived ES (ntES) cell lines [15] previously established in our laboratory
using Sertoli cells of 129B6F1 background with GFP ・・・as donor for nuclear transfer, ・・・.
Male 129B6F1 mice expressing GFP were generated by mating female 129/Sv mice with male C57BL/6 GFP transgenic mice
(double-transgenic mouse line [16] constructed using pCAG-EGFP [10, 11] and Acr3-EGFP [17]).
