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Now we walked over to Vacanti’s lab to see the results. He wasn’t optimistic. Before my arrival, he told me, he had called an emergency phone meeting with Kojima and his brother Martin. “I said, ‘We have to have something to demonstrate that this is indeed real or we’re going to look really stupid.’ ” As it happened, he said, just a few days before, he had seen the best-looking spheres in many months. They had tested positive for the embryonic-stem-cell markers Oct4 and Nanog, calculated by a machine that is not subject to the vagaries of auto-fluorescence. He had broken the spheres apart and placed the cells in a medium that encouraged the growth of neurons. The new plan was to test them for a protein called nestin, which, he said, would show that a mature skin cell had been transformed into a neural stem cell—taken back down to the root and up another branch.
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"Compact and taciturn, Kojima looked up and said “Good data” before saying hello. The nestin levels were fifty to sixty times as high as the last time he had checked. “Ha!” Vacanti said, jumping up and down and cheering.
“It’s only one sample,” Kojima said.
“Doesn’t matter,” Vacanti replied cheerfully. “One’s good for me.”
"
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In the progress of their research, they were all the way back to 2008, before Obokata, but Vacanti still saw a cause for celebration. After calling his brother, he decided that it was time for lunch and a glass of wine; Kojima came along, and Vacanti kept exhorting him to make merry. “I’m excited,” Kojima said. “But I need correct data, otherwise—” He chopped his hand through the air.
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Vacanti was reluctant to ruin a good day, but eventually he couldn’t put off looking at the teratoma slides any longer. Back in the lab, he sat down at the microscope and examined them for a long time. I stole a glance: a view of pink bubbles and marbled swirls like the endpapers of a seventeenth-century Venetian book. After a while, Vacanti concluded that the cells had failed to form new tissues; the only tissues evident had clearly come from the host mouse. Months of work, without producing evidence of pluripotency. But he didn’t dwell on that troubling thought for long. “Koji, you know what I was thinking?” he said. “We had Oct4, we had Nanog, we had nestin. Let’s never do another test. Let’s let it be someone else’s problem!”
ヴァカンティは最後に<Let’s let it be someone else’s problem!>と言ったと
デイナは書き留めていて、その実現が小保方さんのHPであるSTAP HOPE PAGEだわね。
そしてその上でヴァカンティは特許申請を推し進めることにした。それは利権でも
あると同時にその手続きを通して自分たちの捏造疑惑を払拭する手段でもあるのね。
勿論だよ。平たいところで実験したら増殖してシート状になる。それを
PGAに来るんでくちゃくちゃに丸めて皮下に埋めてるだけだから体細胞は
何らかの形で増殖しているさ。ヴァカンティが自分の目で確認したという
体細胞がそれだ。デイナは彼らがその時に使った細胞は皮膚の細胞と書いている。
でも、the only tissues evident had clearly come from the host mouse.だった。
これってドナー細胞の皮膚もあったんじゃないかな。ここのところはデイナ自身に
基礎知識が不足しているからはっきりしないね。
彼の<「STAP細胞事件」-STAP細胞もキメラもSTAP幹細胞もFI幹細胞も全てある>の
コメント欄のやり取りに関しては我々は東大はミトコンドリアの詳しい検証は
していないと思っている。
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Re: No title
FLSのミトコンドリアDNAがB6由来だったという話もありましたね。129B6だとミトコンドリアDNAは129由来になるはずで、だから核移植ではないかと疑われたと思います。これについては別の可能性もあると思っています。
2017.03.11 Sat l wamoga. URL l 編集
No title
・FLSは核移植ES細胞と予想して核移植ES細胞の全ゲノムを読んだが、どうやらFLSは核移植ES細胞ではなさそうだ。
・そこですでに得られたゲノムのデータでSTR解析をおこなった。
・受精卵ES細胞のFES1のみがFLSと一致した。
(日経サイエンス3月号の要約)
よくあることで珍しくはないんだけど、時が経過してもこんなにこじれてしまったのには別の
経緯があるのよね。
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Obokata resisted working on the stem-cell line; she wanted to remain focussed on the research she had been doing. When Wakayama pressed her, she grew embittered. In Boston, Kojima heard her screaming while reading her e-mail. He recalls that she shouted at the computer screen, “No! I don’t want to do it!” Kojima asked her what was going on. “Dr. Wakayama e-mailed me so many times,” she told him. “Like, ‘Did you do this assay? Did you do this experiment?’ Anything related to the stem-cell line, he forces me to do. I don’t want to, because I don’t know how to make it. I tried. I couldn’t.” In spite of their differences, Obokata writes, when Wakayama was offered a position at the University of Yamanashi he urged her to go with him.
和モガさんは丹羽さんが
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Klf4 expression was detected in all samples, which may reflect its expression in liver cells, and thus serves as a positive control in this assay.