Data should be provided on the frequency and reproducibility of in vitro differentiation.
How many cells were grafted for the teratoma assy? Why were NOD/Scid rather than syngeneic mice used as recipients?
Why were NOD/Scid rather than syngeneic mice used as recipients?
The frequency and sizes(weights) of Teratomas should be presented.
Are they teratomas or teratocarcinomas (easily monitored via Oct4-gfp)?
もう一度、アーティクルのテラトーマ作製記述を貼るわよ。
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1 × 107 STAP cells were seeded onto a sheet composed of a non-woven mesh of polyglycolic acid fibres (3 × 3 × 1 mm; 200 μm in pore diameter), cultured for 24 h in DMEM + 10% FBS, and implanted subcutaneously into the dorsal flanks of 4-week-old mice. In this experiment, to better support tumour formation from slow growing STAP cells by keeping cells in a locally dense manner, we implanted STAP cells with artificial scaffold made of polyglycolic acid fibres. Given the artificial nature of the material, we used NOD/SCID mice as hosts, to avoid possible enhancement of post-graft inflammation caused by this scaffold even in syngenic mice. STAP stem cells were dissociated into single cells and cell suspension containing 1 × 107 cells was injected into the testis. Six weeks later, the implants were analysed using histochemical techniques.
ここで分かることは<to avoid possible enhancement of post-graft
inflammation caused by this scaffold even in syngenic mice.という言葉が
論文に入ったのはこのネイチャー査読者への対応なのね。
Why were NOD/Scid rather than syngeneic mice used as recipients?に対する
答えだわね。
これね。第一査読者のコメントの中よ。
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I believe that the green transformation is indeed due to stress as reporters are often upregulated in stressed or dying cells. But the cells that go green may not be the ones in the later green colonies. I think these are more likely to be ES cells acquired by cross contamination and selected for growth by the B27-LlF medium. This would explain the results on marker expression, promoter demethylation, differentiation, and chimera formation.
