キメラができて初めてその細胞が多能性細胞であったということになるんで、何が
キメラになったのかが分からないのに分かってないものを分からないままにいくら
分析しても意味ないよね。T細胞がキメラになったとは証明されていない。T細胞は
実際FACSで分画されて酸浴細胞になってるんだけどね。
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The emergence of Oct4-GFP+ cells at the expense of CD45+ cells was also observed by flow cytometry (Fig. 1c, top, and Extended Data Fig. 1b, c). We next fractionated CD45+ cells into populations positive and negative for CD90 (T cells), CD19 (B cells) and CD34 (haematopoietic progenitors), and subjected them to low-pH treatment. Cells of these fractions, including T and B cells, generated Oct4-GFP+ cells at an efficacy comparable to unfractionated CD45+ cells (25–50% of surviving cells on day 7), except for CD34+ haematopoietic progenitors, which rarely produced Oct4-GFP+ cells (<2%; Extended Data Fig. 1d).
