ホームズ。これはFI-SCの実験ではないんだぜ。STAP細胞の胎盤貢献実験だ。
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When splenic CD45+ lymphocytes are exposed to pH 5.7 for 30 min and subsequently cultured in the presence of LIF, a substantial portion of surviving cells start to express the pluripotent cell marker Oct4 (also called Pou5f1) at day 2. By day 7, pluripotent cell clusters form with a bona fide pluripotency marker profile and acquire the competence for three-germ-layer differentiation as shown by teratoma formation.
それだけじゃないわ。アーティクルでも出会っている。ワンセンテンスごとに
小保方さんと肩を並べて添削していったんだわ。
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We tested the following three different genetic backgrounds of mice for STAP stem-cell establishment from STAP cell clusters, and observed reproducible data of establishment: C57BL/6 carrying Oct4-gfp (29 of 29), 129/Sv carrying Rosa26-gfp (2 of 2) and 129/Sv × C57BL/6 carrying cag-gfp (12 of 16).
a bは若山氏が違うといった？胎児胎盤の写真
c STAP細胞はキメラの胎盤・卵黄のう形成に寄与するとの棒グラフ（キメラ胎児の６割に貢献）
d STAP細胞は胎児形成能を持つOct4,Nalog,Rex1を発現するが、ESとの比較で示す
およびTSマーカー（Cdx2、Eomes, Elf5）の発現についてTSとの比較で示す
e STAP 幹細胞3種は、胎盤形成に寄与しない。 TSとのGFP細胞の割合の比較
f STAP 幹細胞、ES 細胞は Cdx2、Eomes,、Elf5 マーカーを発現しない。TSとの比較
泥縄ではあるけど、Gの意味が分からない。上のグロビンという説明があったような。
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CAG promoter
Hybrid construct consisting of the cytomegalovirus (CMV) enhancer fused to the chicken beta-actin promoter.
そういう泥縄の知識を前提にしてレター論文を読む。そこには(Fig. 1a; shown with Rosa26-GFP)とある。
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Here we have investigated the unique nature of STAP cells, focusing on their differentiation potential into the two major categories (embryonic and placental lineages) of cells in the blastocyst. We became particularly interested in this question after a blastocyst injection assay revealed an unexpected finding. In general, progeny of injected ES cells are found in the embryonic portion of the chimaera, but rarely in the placental portion (Fig. 1a; shown with Rosa26-GFP). Surprisingly, injected STAP cells contributed not only to the embryo but also to the placenta and fetal membranes (Fig. 1b and Extended Data Fig. 1a–c) in 60% of the chimaeric embryos (Fig. 1c).
アーティクル論文を読む。そこには<129/Sv carrying Rosa26-gfp (2 of 2)>がある。
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We tested the following three different genetic backgrounds of mice for STAP stem-cell establishment from STAP cell clusters, and observed reproducible data of establishment: C57BL/6 carrying Oct4-gfp (29 of 29), 129/Sv carrying Rosa26-gfp (2 of 2) and 129/Sv × C57BL/6 carrying cag-gfp (12 of 16).
