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Both scientists were speaking to Bioscience Technology about Wakayama’s recent discovery that two of several STAP stem cell batches made for two embattled Nature papers were apparently not derived from a 129 mouse strain, as Wakayama was told. They were derived from F1 and B6 mouse strains.
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“This is just preliminary data, but strong data,” emailed Wakayama, an author on both Nature STAP papers. “Very sad results. However, that 129 mouse experiment data was not used for [primary conclusions of the] Nature papers. We must wait until final data comes from others.”
[ ]内は君の文章を記者が補っただけなんだろうが、そもそも君はいつから調査隊に入ったんだい。君は容疑者だぜ。
しかも責任著者だ。小保方さんや他の共著者に言わないで誰に通報しているんだい。Very sad results.が聞いて飽きれる。
お前が行った実験だろう。[ ]内が無いと、事実として論文に書いてあることと矛盾するから記者が補った。
実際はどうなんだい。返ってきたマウスがどうであったか以前に、渡したマウスはただのCAG-GFPなのかい、
それともRosa26-gfpだったのかい。君は答えていない。
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The erroneous 129 data landed in one of the papers, contrary to press reports, Wakayama says. (See below.) The data indeed do not affect the papers’ main thrust, but have spurred calls for closer re-evaluations of other STAP stem cell lines. Wakayama has been analyzing other lines. So far, he says, "only the 129 mouse data are not true."
なにがThe data indeed do not affect the papers’ main thrust, だい。Rosa26-gfpが
光ってるんだったらTCR再構成証明は不要なんだぜ。馬鹿な事言ってんじゃねえや。
"only the 129 mouse data are not true."って、お前は12012年11月末に小保方さんから
12/11ヴァージョン原稿を受け取った時からそこに129/Sv carrying Rosa26-gfp (2 of 2)ち
あったのを1年以上放置してたんだぞ。今更何がそこだけ間違いだ。忘却したんなら桃子に
何を言ったか確認してこい。
この下準備に情報流出したのはお前だよな。
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But in ensuing weeks, blogger scientists—notably Juuichi Jigen in Japan, Paul Knoepfler in the US, and anonymous PubPeer commenters--have uncovered problems with the papers. Four investigations were launched by Riken, Harvard, Nature, and the university that approved the PhD thesis of first author Haruko Obokata, Waseda. The most serious problems have included irreproducibility, and two sets of staining images that were supposed to prove pluripotency—but instead appeared to come from Obokata’s unrelated thesis, likely rendering them useless.
129背景でもできるかどうかを試すときにどうしてこれをRosa26-gfpだと言ったのかい?それとも
言ってないのに小保方さんがそう書いて、笹井さんと丹羽さんの質問に若山さんがそういったと
言わなかったとでも思ってるのか?
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Then, a few days ago, Wakayama finished a genetic analysis of two batches of STAP stem cells he made from STAP cells Obokata said came from the 129 mouse strain, and discovered another problem. For the papers, Wakayama had asked Obokata to make STAP cells from 129 mice so he could be sure the ability to dedifferentiate was not confined to the papers’ main mouse strain (B6), he says.
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Obokata gave him two batches of STAP cells she said she made from 129 cells. But recent developments led him to analyze the stem cells he developed from them. They didn’t come from 129.
Rosa26-gfpの記述が抜けてるぞ。ストレーンの問題にすり替えるなよ。
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“In my field, it is very important to show the same results in different strains,” Wakayama said via email. “For example, cloned mice can be easily generated from F1 mice. But it is too difficult to clone from many inbred strains, such as B6—except for the 129 mouse. “
The 129 strain was briefly mentioned, in both the first Nature paper, and the March 5 revised protocol of Riken’s Hitoshi Niwa (another respected stem cell scientist and co-author). In both, it was noted “reproducible establishment” of STAP cells occurred with B6, F1, and 129. “STAP stem cells with all these genetic backgrounds formed chimaera-forming activity,” both read.
お前は12/11ヴァージョンで笹井さんのリヴァイズ以前からRosa26-gfp記載があることを
知っていたと桃子に証言されてしまっている。
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The latter sentence seems to be an additional, if simple, “mistake,” Wakayama said via email. He made stem cells from the STAP cells she said came from 129 mice. But he never made chimeras from the 129 cells, as stated in the paper (and Niwa protocol). “I gave 129 mice to Dr. Obokata. Then, Dr. Obokata generated STAP cells from those mice, and gave me two STAP cells. I do not know how many STAP cells were generated at that time. I got just two. Then, I established two STAP stem cell lines from those two STAP cells. However, I did not make chimera from those cell lines. Then I left RIken." In another email he clarified: "I left Riken one year ago. Then we could not (establish) contact well between authors. Maybe Dr. Sasai, who wrote the papers, thought that I did chimera experiment of 129.”
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Next Steps
Asked about the new 129 mouse developments, a Riken spokesperson emailed: “We have received genetic analysis data on STAP stem cells from Professor Wakayama. However, this is still preliminary data, and we will cooperate with Professor Wakayama with regard to more detailed testing.”
