(英文)
a, Immunostaining for pluripotent cell markers (red) in day 7 Oct4-GFP+ (green) clusters. DAPI, white. Scale bar, 50 μm. b, qPCR analysis of pluripotency marker genes. From left to right, mouse ES cells; parental CD45+ cells; low-pH-induced Oct4-GFP+ cells on day 3; low-pH-induced Oct4-GFP+ cells on day 7. n = 3; error bars show average ± s.d. c, DNA methylation study by bisulphite sequencing. Filled and open circles indicate methylated and non-methlylated CpG, respectively. d, Immunostaining analysis of in vitro differentiation capacity of day 7 Oct4-GFP+ cells. Ectoderm: the neural markers Sox1/Tuj1 (100%, n = 8) and N-cadherin (100%, n = 5). Mesoderm: smooth muscle actin (50%, n = 6) and brachyury (40%, n = 5). Endoderm: Sox17/E-cadherin (67%, n = 6) and Foxa2/Pdgfrα (67%, n = 6). Scale bar, 50 μm.
つぎはbね。
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b, qPCR analysis of pluripotency marker genes. From left to right, mouse ES cells; parental CD45+ cells; low-pH-induced Oct4-GFP+ cells on day 3; low-pH-induced Oct4-GFP+ cells on day 7. n = 3; error bars show average ± s.d.
参考
調査報告
９）Article Fig.2b、3d、3g、Extended Data Fig.1a、Extended Data Fig.6dについて
エラーバーが不自然である点
（調査結果）
本件に関しては、Extended Data Fig.1aについて過去に投稿された論文原稿に遡りグラフが投稿毎に一致しないこと（特にエラーバーのサイズ、有無など）を確認した。小保方氏本人対する聞き取り調査で、図を描画ソフト等で修正したことはないかを含め原因の心当たりを確認したところ、修正したことはないが、本人としてもエラーバーが不自然であること、ただ表計算ソフトの問題でこのようなことはよく起きると考えていたとの回答を得た。パソコンに入っていると思われるオリジナルデータの提出を小保方氏に求めたが、提出されなかった。
Science査読
Reviewer 1
In Fig.2B and the other RT-PCR studies, it is not stated whether the Y-axis is linear or logarithmic. If it is linear, which seems more likely, then I am very surprised that all of the pluripotency genes measured in the ESC control have virtually the same RNA abundance, which exceeds that of GAPDH.
Reviewer 2
Regarding the existing molecular data on the identity of the cell lines, the embryonic gene expression qPCRs (Figure 2B, S3C) show unusually high values for expression levels relative to GAPDH levels. Even though the figure has an ESC control, and it may be a primer-specific phenomenon, mRNA levels of genes such as Nanog and Rex1 are more like 0.05 or 0.15 of GAPDH levels, whereas the authors observed levels as high as 12 -14 times GAPDH.
まだ最初のあなたの昨日の指摘まで行ってないよ。あなたの昨日の指摘はdでしょ。
そんなにどんどん先走らないでください。
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d, Immunostaining analysis of in vitro differentiation capacity of day 7 Oct4-GFP+ cells. Ectoderm: the neural markers Sox1/Tuj1 (100%, n = 8) and N-cadherin (100%, n = 5). Mesoderm: smooth muscle actin (50%, n = 6) and brachyury (40%, n = 5). Endoderm: Sox17/E-cadherin (67%, n = 6) and Foxa2/Pdgfrα (67%, n = 6). Scale bar, 50 μm.
