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Figure 5: アnalyses of fluorescent signals from GOF transgene.
Figure 5
(a) Fluorescent microscopic アnalysis of cell aggregates derived from GOF Tg mice. The cell aggregates (top) were derived from liver cells of 6-days old of GOF Tg mice. Fluorescent images with the filter sets for detection of GFP and RFP signals are shown. Images of ES cells carrying CAG-GFP (middle) captured with the same conditions are shown as a control to confirm no leaky signal of GFP in RFP channel. Images of wild-type ES cells (bottom) are shown as a control to confirm spesific signal of GFP in GFP channel. (b) FACS analysis of the low-pH treated spleen cells derived from GOF Tg mice. The spleen cells were isolated from 7-day-old GOF Tg mice and prepared with Lympholyte followed by treatment with the indicated stressors. After the culture for seven days, the cells were dissociated, stained with anti-E-cadherin with PE and anti-CD45 with APC, and analyzed by FACS. Wild-type ES cells were used as a positive control for E-cadherin staining and a negative control for CD45-staining as well as GFP fluorescence.
