129B6F1G1 and GR14 are nuclear transfer-
derived ES(ntES) cell lines (Wakayama et al
2001) previously established in our laboratory
using sertoli cell from a 129B6F1 background
With GFP(Ohta ＆ Wakayama 2005) and
tail tip cell of a mail mous
(129BDF2. Wakayama et al 2005) respectively
as donor for nuclear transfer karyotype
analysis revealed that the ES cell lines
Used had the normal karyotype at the
following percentages (number of metaphases
with normal karyotype in parentheses)
46%(12／26)for E14 35%(9／26) for
129B6F1G1 and54%(14／26)for GR14
①2005年論文(これが太田さんのntESを作った時の実験。ただし、Lさんのおっしゃる通り、この論文自体はクローンの実験。)
Generation of Normal Progeny by Intracytoplasmic Sperm Injection Following Grafting of Testicular Tissue from Cloned Mice That Died Postnatally
Hiroshi Ohta Teruhiko Wakayama
Biology of Reproduction, Volume 73, Issue 3, 1 September 2005, Pages 390–395, doi.org/10.1095/biolreprod.105.041673
Published: 01 September 2005
②2008年8月論文(坂出さんとの共著論文)
Generation of mice derived from embryonic stem cells using blastocysts of different developmental ages
Hiroshi Ohta, Yuko Sakaide and Teruhiko Wakayama
Received 30 April 2008
Revision requested 28 May 2008
Revision received 15 July 2008
Accepted 28 August 2008This Article
Published online before print August 29, 2008, doi: 10.1530/REP-08-0184
③2008年9月論文(特許関連論文)
Increasing the Cell Number of Host Tetraploid Embryos Can Improve the Production of Mice Derived from Embryonic Stem Cells
Hiroshi Ohta Yuko Sakaide Kazuo Yamagata Teruhiko Wakayama
Biology of Reproduction, Volume 79, Issue 3, 1 September 2008, Pages 486–492, doi.org/10.1095/biolreprod.107.067116
Published: 01 September 2008
この論文のマテメソに、以下のようにあるわ。
>>
The 129B6F1G1 [13] and BDmt2 [14] are nuclear transfer-derived ES (ntES) cell lines [15] previously established in our laboratory using Sertoli cells of 129B6F1 background with GFP and tail-tip cells of a male BDF1 mouse as donor for nuclear transfer, respectively. Male 129B6F1 mice expressing GFP were generated by mating female 129/Sv mice with male C57BL/6 GFP transgenic mice (double-transgenic mouse line [16] constructed using pCAG-EGFP [10, 11] and Acr3-EGFP [17]).
あなたが引用したのは②の論文の以下ね。
>>
129B6F1G1 and GR14 are nuclear transfer-derived ES (ntES) cell lines (Wakayama et al. 2001) previously established in our laboratory using Sertoli cells from a 129B6F1 background with GFP (Ohta & Wakayama 2005) and tail tip cells of a male mouse (129BDF2; Wakayama et al. 2005) respectively, as donors for nuclear transfer. Karyotype analysis revealed that the ES cell lines used had the normal karyotype at the following percentages (number of metaphases with normal karyotype in parentheses): 46% (12/26) for E14; 35% (9/26) for 129B6F1G1; and 54% (14/26) for GR14.
ここで<論文に書かれていたのはクレアの129+Ter>だと言ってるのは2005年論文のことね。
マテメソに書かれている。Terだわね。
>>
To generate 129B6F1 mice carrying the GFP transgene, female 129/Sv-ter mice were mated with male C57BL/6 GFP transgenic mice, and the offspring of these matings, which were hemizygous for the GFP transgene, were used as donors for nuclear transplantation.
それはむしろ2008年論文のために作成されたntESじゃないかな。というのも
例えば2008/8月論文には履歴があるよね。
Received 30 April 2008
Revision requested 28 May 2008
Revision received 15 July 2008
Accepted 28 August 2008
論文が提出されたのは4/30でアクセプトされたのが8/28だ。4か月かかっている。
論文のための実験はそれ以前に行われているんだから2007/8/7凍結というのは
論文提出10か月前だよ。小保方さんの実験なんて2年がかりなんだからね。この
G1が特許関連論文のみかぃとだというのは時間的な不整合はないね。
