129B6F1G1 and GR14 are nuclear transfer-
derived ES(ntES) cell lines (Wakayama et al
2001) previously established in our laboratory
using sertoli cell from a 129B6F1 background
With GFP(Ohta ＆ Wakayama 2005) and
tail tip cell of a mail mous
(129BDF2. Wakayama et al 2005) respectively
as donor for nuclear transfer karyotype
analysis revealed that the ES cell lines
Used had the normal karyotype at the
following percentages (number of metaphases
with normal karyotype in parentheses)
46%(12／26)for E14 35%(9／26) for
129B6F1G1 and54%(14／26)for GR14
