そこにまた小保方さん達の論文の書き方にも曖昧さがあったのよね。
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Chimaeric mouse generation and analyses
For production of diploid and tetraploid chimaeras with STAP cells, diploid embryos were obtained from ICR strain females. Tetraploid embryos were produced by electrofusion of 2-cell embryos. Because trypsin treatment of donor samples turned out to cause low chimaerism, STAP spherical colonies were cut into small pieces using a microknife under the microscope, and small clusters of STAP cells were then injected into day-4.5 blastocysts by a large pipette. The next day, the chimaeric blastocysts were transferred into day-2.5 pseudopregnant females. For experiments using STAP cells from CD45+ cells without the Oct4-gfp reporter, STAP cell clusters were identified by their characteristic cluster morphology (they are made of very small cells with no strong compaction in the aggregate). When the STAP conversion conditions (low pH) were applied to CD45+ lymphocytes, most day-7 clusters that were large and contained more than a few dozen small cells were positive for Oct4 (although the expression level varied).
ついでだからもう一度全部ね。
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Therefore, we used only well-formed characteristic clusters (large ones) for this type of study and cut them by microknife to prepare donor cell clusters in a proper size for glass needle injection. For an estimate of the contribution of these injected cells, we used STAP cells that were generated from CD45+ cells of mice constitutively expressing GFP (C57BL/6 line with cag-gfp transgenes; F1 of C57BL/6 and 129/Sv or DBA/2 was used from the viewpoint of heterosis).
続き
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Because the number of CD45+ cells from a neonatal spleen was small, we mixed spleen cells from male and female mice for STAP cell conversion. To make germline transmission more efficient, we intercrossed chimaeras in some experiments.
For the production of diploid and tetraploid chimaeras with STAP stem cells, diploid embryos were obtained from ICR strain females. Tetraploid embryos were produced by electrofusion of 2-cell embryos. STAP stem cells were dissociated into single cells and injected into day-4.5 blastocysts. In the chimaera studies with both STAP cells and STAP stem cells, we did not find tumorigenetic tendencies in their chimaeras or their offspring (up to 18 months).
この<To make germline transmission more efficient, we intercrossed
chimaeras in some experiments.>の部分は一番最後の(up to 18 months).の
後ろに付くべき文章なのではないかということね。気持ちはSTAP細胞だけでなく、
STAP幹細胞の実験にまで及んでいるので、そもそもSTAP細胞のジャームライン
トランスミッション実験では兄妹交配だけしか行われていない。それに対して
幹細胞の実験ではどういう交配であったのかは書かれていない。ただ、
<To make germline transmission more efficient, we intercrossed
chimaeras in some experiments.>と言う文章でSTAP幹細胞側の実験では
兄妹交配でない実験もあるということを示唆しているのね。<in some experiments>
と言う句は全部兄妹交配なら入れる必要の無い句で、この句に若山さんが
小保方さんに言った句言葉の影響が示されているのよね。
