三誌論文の最後のサイエンス論文の概要がだんだん明らかになりつつあるわね。
第2レフェリーの纏めの前に、第3レフェリーのコメントは少ないんで、先に
こちらをやっつけてからサイエンス論文全体がどういう趣旨だったかを推定してみましょうかね。
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Reviewer 3
The finding described in this manuscript is very unusual and unexpected. Under certain circumstances, it appears that a non-physiological non-specific stress can trigger reprogramming of terminally differentiated cells, such that the cells enter a pluripotent stem cell-like state.
今日はボランティアの所用ね。あんまり時間はないのね。はい、次よ。
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If these results are repeatable, a paradigm of developmental biology would be changed. Currently, that paradigm is that terminally differentiated states are set and cannot be reset.
2010年に既にムー論文が出ているんで、Currently, that paradigm is that
terminally differentiated states are set and cannot be reset.というのは
皮肉を言うためのレトリックなんだよな。事実じゃない。
桃子が如何に資料を我田引水しているかがわかる。桃子は信じやすい人間なんだよな。
一旦バイアスが掛かると自己修正できない。ニーチェが歴史的事象に関して、
昆虫のように複眼視しろとアドヴァイスしているのを知らないんだろうな。
訓練されていないジャーナリストだな。
はい。次よ。サクサク行かないと10時になっちゃうわ。
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Although Yamanaka broke this biological rule by overexpressing pluripotency-associated factors, that system is highly artificial.
はい。次よ。
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The authors of the present manuscript propose that cells have an intrinsic capacity to reprogram. I found the manuscript to be clearly written and concise, although sometimes mildly unorthodox in terms of literature cited.
はい、はい、やっとその本題よ。
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For example, the section on Oct4 should state how many cells were sorted and describe the appearance of the cells. Is it possible that rare populations of cells pre-exist or are already apparent on day 1 (thus, what are the “dots” of Fig. 1?). The authors will argue that, indeed, under certain circumstances, they were able to reprogram terminally differentiated cells, and that this was attributable to TCR recombination. I think, ideally, that the cells should be experimentally tagged and traced. This would unequivocally clarify the source of the cells and, further, would exclude the possibility that some cells pre-existed in a pluripotent state.
はい、次ね。
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Critically, it is necessary to determine whether SAC cells can propagate stably in culture and whether such cells can be passaged. CD45.2 cells from the spleen are differentiated and, unless activated by an antigen, are supposed to be in G0. Do these cells re-enter the cell cycle? The cells should be further characterized.
