キメラのところというより、幹細胞のところでしょ。ここでしょ。
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STAP stem-cell conversion culture
For establishment of STAP stem-cell lines, STAP cell clusters were transferred to ACTH-containing medium36 on MEF feeder cells (several clusters, up to a dozen clusters, per well of 96-well plates). Four to seven days later, the cells were subjected to the first passage using a conventional trypsin method, and suspended cells were plated in ES maintain medium containing 20% FBS. Subsequent passaging was performed at a split ratio of 1:10 every second day before they reached subconfluency. We tested the following three different genetic backgrounds of mice for STAP stem-cell establishment from STAP cell clusters, and observed reproducible data of establishment: C57BL/6 carrying Oct4-gfp (29 of 29), 129/Sv carrying Rosa26-gfp (2 of 2) and 129/Sv × C57BL/6 carrying cag-gfp (12 of 16). STAP stem cells with all these genetic backgrounds showed chimaera-forming activity.
Company name
chromocenter Inc.
Capital
238,706,000 yen
Representative Director and President
Takashi Matsuoka
Headquarters
133-2, Nishicho, Yonago-shi, Tottori.
TEL:0859-37-3838 FAX:0859-37-3833
Yonago research center
TOTTORI Bio FRONTIER 3F,86, Nishicho, Yonago-shi, Tottori
Kobe research center
HI-DEC 4F, 6-7-4, Minatojimaminami-machi, Chuo-ku, Kobe, Hyogo
Tokyo office
Hayashiya Bldg room 302 , 1-4, Nihonbashiyokoyamacho, Chuo-ku, Tokyo
TEL:03-5623-2831
business content
Construction of a stable gene expression cell line
Development of a protein high production course
Chromosome analysis service
