ここだね。
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CD45+ cell-derived STAP cells contributed to all tissues examined (Fig. 4d). Furthermore, offspring derived from STAP cells were born to the chimaeric mice (Fig. 4e and Extended Data Fig. 7c), demonstrating their germline transmission, which is a strict criterion for pluripotency as well as genetic and epigenetic normality32, 33. Furthermore, in a tetraploid (4N) complementation assay, which is considered to be the most rigorous test for developmental potency34, 35 (Fig. 4a, bottom), CD45+ cell-derived STAP cells (from F1 mice of B6GFP × 129/Sv or DBA/2) generated all-GFP+ embryos on embryonic day (E)10.5 (Fig. 4f, Extended Data Fig. 7d and Supplementary Video 3), demonstrating that STAP cells alone are sufficient to construct an entire embryonic structure. Thus, STAP cells have the developmental capacity to differentiate into all somatic-cell lineages as well as germ-cell lineages in vivo.
STAP細胞はES細胞よりやや小さいんだね。でもES細胞とSTAP幹細胞は区別がつかない。
STAP細胞が小さいという認識は最初のころは胞子様細胞は小さく存在していて
体細胞内で休眠しているという発想だった。博論草稿にあるよね。
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The in vitro behavior of cells in the spheres is likely to be very different from cells that reside in vivo. How these stem cells harbor in adult body and how they exert their potential
letter論文の引用文献
*ttps://www.ncbi.nlm.nih.gov/pubmed/2598811
「An assessment of the developmental potential of embryonic stem cells in the midgestation mouse embryo.」
Embryonic stem cells (ES) cells were injected into host blastocysts either in groups of 10-15 cells or as single cells in order to test their developmental potential in the developing embryo. The analysis of midgestation chimaeras, by electrophoretic separation of glucose phosphate isomerase (GPI) isozymes, showed that ES cells were capable of colonizing trophectoderm and primitive endoderm derivatives at a low frequency, as well as producing a high rate of chimaerism in tissues of the fetus and extraembryonic mesoderm.
