今ふむ氏と翻訳機君たちが時間を見ては何かこそこそやってるようだけど、
丹羽さんはあの論文であんなに見事に光っている細胞塊を作れていない。そもそも
彼は自分ではなかなかできなかったんだよね。それで小保方さんにいろいろとコツを聞いて
やっとあの肝細胞からのスフィア作製に成功している。
based on personal communication with the authors of the original study.
とあるよね。
ここね。
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Next we examined the effect of adenosine triphosphate (ATP) as a transient low-pH stressor based on personal communication with the authors of the original study. The amount of the diluted ATP solution to achieve optimized low-pH (~5.7) was adjusted(Fig. 1a) and experiments were repeated several times. Massive cell death was again observed at two days after treatment (Fig. 2a); however, we found that liver cells reproducibly gave rise to cell aggregates morphologically similar to those shown in the previous report, whereas spleen and heart cells only occasionally formed similar cell aggregates (Fig. 1b). The efficiency of aggregate formation was clearly higher for ATP-treated cells than for HCl-treated or non-treated cells, especially in the case of liver cells.
丹羽論文のここにRFPという言葉が出で来るんだけど、李がどういうつもりの嘘をついているのかというあたりだな。
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Figure 5: アnalyses of fluorescent signals from GOF transgene.
Figure 5
(a) Fluorescent microscopic analysis of cell aggregates derived from GOF Tg mice. The cell aggregates (top) were derived from liver cells of 6-days old of GOF Tg mice. Fluorescent images with the filter sets for detection of GFP and RFP signals are shown. Images of ES cells carrying CAG-GFP (middle) captured with the same conditions are shown as a control to confirm no leaky signal of GFP in RFP channel. Images of wild-type ES cells (bottom) are shown as a control to confirm spesific signal of GFP in GFP channel. (b) FACS analysis of the low-pH treated spleen cells derived from GOF Tg mice. The spleen cells were isolated from 7-day-old GOF Tg mice and prepared with Lympholyte followed by treatment with the indicated stressors. After the culture for seven days, the cells were dissociated, stained with anti-E-cadherin with PE and anti-CD45 with APC, and analyzed by FACS. Wild-type ES cells were used as a positive control for E-cadherin staining and a negative control for CD45-staining as well as GFP fluorescence.
