<南北首脳：戦争終結へ、完全な非核化目指す－歴史的な「板門店宣言」>
Bloomberg
2018/04/28 00:56
<核のない朝鮮半島を実現するという共通の目標>なんて認めてないんでね。
目標ではない。即時撤去しろと命令している。無論米軍が乗り込んで確認する。
当たり前だろ。
原文にこうある。 Kanga Kongが工作員だな。
By Kanga Kong and Andy Sharp
2018年4月27日 17:58 JST Updated on 2018年4月27日 21:57 JST
はい、どうぞ。
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a, Culture of Oct4-GFP Fgf4-induced cells in LIF + 20% FBS medium. b, qPCR analysis of ES-like cells derived from Fgf4-induced cells for pluripotent marker genes (left) and trophoblast marker genes (right). Values are shown as ratio to the expression level in ES cells (left) or trophoblast stem (TS) cells (right). c, d, Culture of Oct4-GFP Fgf4-induced cells sorted by FACS for strong integrin α7 (Itga7) expression in LIF + 20% FBS medium. d, Formation frequency (shown by percentage) of Oct4-GFP+ colonies from cells plated on gelatin-coated dishes at a clonal density. **P < 0.01; t-test; n = 3. e, f, Culture of Oct4-GFP Fgf4-induced cells (dissociated) in LIF + 20% FBS medium with MEK inhibitor. **P < 0.01; NS, not significant; Tukey’s test; n = 3.
続きよ。
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e, No substantial formation of Oct4-GFP+ colonies was seen from Fgf4-induced cells in the presence of MEK inhibitor (left), whereas colonies frequently formed when cells were co-plated with Oct4-GFP ES cells (right; plated cells were 1/20 of Fgf4-induced cells). f, Quantification of colony formation per plated cells (1 × 103 Fgf4-induced cells and/or 1 × 103 ES cells). Unlike Fgf4-induced cells, ES cells formed colonies (regardless of co-plating with FI-SCs) in the presence of MEK inhibitor. Bars and error bars represent mean values and s.d., respectively (b, d, f). Scale bars: 100 μm (a, c, e).
ここだわ。
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To confirm further that Fgf4-induced stem cells with a trophoblast-like nature were converted into ES-like cells, rather than just selecting ES-like cells pre-existing in the Fgf4-induced stem cell culture, we examined the effect of the MEK inhibitor PD0325901 on the ES-like cell generation from Fgf4-induced stem cells. Like trophoblast stem cells, Fgf4-induced stem-cell survival is dependent on FGF–MEK signals, and the inhibition of MEK activity caused massive cell death (Extended Data Fig. 6c). However, PD0325901 is also known to be a main effector in 2i medium[17] and to promote ES cell maintenance. Addition of PD0325901 to LIF+FBS-containing medium strongly inhibited the formation of ES-like colonies from Fgf4-induced stem cells (Fig. 3e, left, and Fig. 3f). This inhibition was unlikely to be due to secondary toxic effects from massive cell death of Fgf4-induced stem cells, as colonies formed in the presence of PD0325901 when ES cells were co-plated in the same culture with Fgf4-induced stem cells (Fig. 3e, right, and Fig. 3f).
