The article also describes teratomas derived from Oct4-GFP STAPcells as evidence for pluripotency (Fig. 2 and Extended Data Fig. 4 inref. 1).
We found a glass slide specimen from which all these teratomaimages were taken, and its corresponding paraffin block.
Quantitative PCR of genomic DNA extracted from this paraffin block reproducibly indicated that these teratoma tissues formed from FES1-derived cells(Fig. 1d, e).
Immunostaining revealed that intestinal epithelium tissue(Fig. 2e, right in ref. 1) and pancreatic tissue (Extended Data Fig. 4c inref. 1),
shown as teratomas from STAP cells1, were GFP-negative and,thus, of host mouse origin (Fig. 1f–h).