日本人は死を見ること帰するが如しだものね。いざとなったら座るのよね。
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4. Like most people, I am convinced that the mouse studies on STAP are solid and convincing. You personally have a top-notch reputation as a scientist around the world. People are instead more specifically concerned about the STAP stem cells themselves. One of the most common questions I am getting asked at this point is this–could the STAP cells have been contaminated with either mESCs or mouse iPS cells? Is that possible? How might that have happened?
まあ、機械のくせに人間様に意見するなんて。
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5. You mentioned that STAP stem cell is not working in your new lab. Why do you think that might be in terms of the methods? What is different now? Also, you mentioned that STAP worked for you when you were at RIKEN. Can you please be a bit more specific? Did you do 100% of the steps in the STAP induction yourself? Again, could iPS cells or ES cells somehow have gotten into the culture?
ふん、殺人機械は兵糧攻めに弱いのよね。そのときは電源切ってやる。
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Teru: I just learned one time from Dr. Obokata, then left RIKEN.
Do you know when we moved to a different lab in the past, how difficult it was then to reproduce even my own techniques? When I moved from Hawaii to Rockefeller, I spent half a year to reproduce cloned mice. This is my technique, but I still needed a lot of time. However, the method of STAP generation is not my technique, therefore, different lab and not my discovered techniques, so this is understandably more difficult to reproduce again.
I did 100% by myself, but each step was looked by Dr. Obokata. Much the same, one of my PhD student also succeeded to establish STAP-SC.
In those early stages of the experiment, we did not culture ES cell nor iPS cell at same time. After that, as control, we sometimes culture ES cell at same time.
サクサク行くんでしょ。メモしとこ。
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①When I succeeded to establish STAP-SC, the original STAP cells expressed Oct4-GFP very much.
②In addition, whole mRNA expression data suggest that STAP-SC are not ES cell.
③I did 100% by myself, but each step was looked by Dr. Obokata.
④Much the same, one of my PhD student also succeeded to establish STAP-SC.
⑤In those early stages of the experiment, we did not culture ES cell nor iPS cell at same time. After that, as control, we sometimes culture ES cell at same time.
ノフラーはSTAP細胞とSTAP幹細胞を論文に書かれているようには厳密に区別してないのよね。
だから彼がSTAP-SCと言ってるときSTAP-cellsと区別されてない。という以前に、論文を
信じてないから、STAP細胞をそのまま幹細胞認識しているのね。これは信じなければ当然の態度ね。
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6. Many people are trying the STAP method around the world and getting frustrated because it isn’t working. I know there is a methods paper in the works, but given how important this is would you consider putting a detailed step-by-step protocol out to the scientific community right now? For example, I’d be happy to post it on my blog and it would have no effect on the publication of a methods paper in a journal. This might really help people to get it to work. Waiting a month or two for a methods paper to come out might be too late.
I know there is a methods paper in the works, は丹羽さんのプロトコルの事じゃなくて
二報の論文の中に書かれているプロトコルを意味しているよね。このブログは2/27付で、
丹羽さんのプロトコル発表は3/5だ。ちなみに遠藤氏の「舐めてますね、これ」は3/9だ。
アーティクル、レターの両方にそれぞれのプロトコルが書かれている部分があるんだから
I know there are methods papers in the works, でないといけないわよね。ノフラーは
アーティクルのSTAPの作り方だけを意識しているのね。
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Teru: Yes, now RIKEN will soon publish the detailed method. I contributed chimera and to establishment. However, chimera and establishment is usual protocol, not specific, because it is the same or more easier than ES cell. Unfortunately, now all responsibility is in RIKEN, and I left RIKEN. Although I want to know but I do not know now.
はい。
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Teru: I do not escape. Because in my results everything is true. However, it is going to take time to reproduce the new technique. For example, the first cloned animal, Dolly, wasn’t reproduced for one and half years after it was published. Human cloned ES cell paper has still not yet been reproduced. Therefore, please wait at least one year. I believe that during this period someone or myself will success to reproduce it.
はい、やっと終りね。注よ。
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Note: At his request, I have made a scattering of small corrections to Teru’s answers just for a few typos/spelling/grammar issues since English is not his first language, but I did not change in any way the tone or significant content of his answers.
こんなものなしら。
①When I succeeded to establish STAP-SC, the original STAP cells expressed Oct4-GFP very much.
②In addition, whole mRNA expression data suggest that STAP-SC are not ES cell.
③I did 100% by myself, but each step was looked by Dr. Obokata.
④Much the same, one of my PhD student also succeeded to establish STAP-SC.
⑤In those early stages of the experiment, we did not culture ES cell nor iPS cell at same time. After that, as control, we sometimes culture ES cell at same time.
⑥However, chimera and establishment is usual protocol, not specific, because it is the same or more easier than ES cell.
⑦I doTherefore, please wait at least one year. I believe that during this period someone or myself will success to reproduce it.
⑧ I do not escape. Because in my results everything is true.
⑨since English is not his first language
