小保方さんはスフィア塊20個に1個Oct4発現があると報告したんだ。
それに対して、丹羽さんは同じ現象を再現確認したうえで、その細胞の数を数えた。
結果は
However, the frequency was very low; 5 × 105 liver cells yielded only ~30 cell aggregates, in which about 20% of the cell aggregates contained 1–2 Oct3/4 positive cells, indicating a frequency per seeded liver cell of 0.0012–0.0024%.
というものだ。105は10の5乗。
①から⑦までの現象ではこの細胞が多能性を持つということはできない。
小保方さんの論文の題は
<ティシュー誌>
The Potential of Stem Cells in Adult Tissues Representative of the Three Germ Layers
<博論>
Isolation of pluripotent adult stem cells discovered from tissues derived from all three germ layers
えっとね。ディスカッションのところでしょ。
>>
However, when we used ATP instead of HCl, also based on a suggestion by the authors, a few cells in a subset of cell aggregates expressed the pluripotency marker Oct3/4 at levels comparable to those in ES cells that were reproducibly detected by QPCR (Fig. 3c) and immunostaining (Fig. 4b).
ここと、
>>
Moreover, since the frequency of Oct3/4-positive cells in the cell aggregates was quite low, it was impossible to determine whether they were selected from the original population or induced in culture, again highlighting the lack of evidence supporting the existence of the reported STAP phenomenon.
だろ。
小町ちゃん、 丹羽さんが again highlighting the lack of evidence
supporting the existence of the reported STAP phenomenon. と言ってるのは
キメラができなかったことを理由にしているね。それから別途相沢さんが
この程度の数ではESですらテラトーマはできないと指摘している。違うかね。
<ティシュー誌>
The Potential of Stem Cells in Adult Tissues Representative of the Three Germ Layers
<博論>
Isolation of pluripotent adult stem cells discovered from tissues derived from all three germ layers