Letterの記載は、「Surprisingly, injected STAP cells contributed not only to the embryo but also to the placenta and fetalmembranes (Fig. 1b and Extended Data Fig. 1a–c) in 60% of the chimaeric embryos (Fig. 1c).」ですね。
で、Extended Data Fig. 1a–cは、「Chimaeric mouse with STAP cells derived from CD45+ cells of B6GFP/129/Sv mice (B6GFP, C57BL/6 line with cag-gfp transgene).」
とありますから、僕のマウス(129/B6/F1のcaf-GFP)で特に矛盾が無かったので、記載しなかったと言うことでは？
Extended Data Fig. 1a–cの画像も同じフォルダーにあったのかは知りませんが。
Quality control method for RNA-seq using single nucleotide polymorphism allele frequency
Fig.4A
Examination of incorporated mRNAs in FI-SCs.(A) Expression of TSC marker genes…
遠藤論文は１割のＴＳコンタミという推測ですね。
>Therefore, the claim in the Obokata et al. paper that FI-SCs contribute to the placenta might have been based in error on contamination by TSCs, which are known to be organ stem cells (Tanaka et al. 1998).
でもこれは、胎盤が光ったかどうかとは無関係かもしれませんよ。というのも
そもそも光ってなければ光るべき何を持って行きようもありません。彼女は
樹形図に都合の良い実験結果が欲しかっただけで、ＦＩ幹細胞を持っていっても
笹井先生の求めるデータは得られないことくらい知っていたでしょうよ。
光ったか否かと、ｍＲＮＡ解析データ提出の動機は分けて考えた方がよさそうです。
>>361
>Letterの記載は、「Surprisingly, injected STAP cells contributed not only to the embryo but also to the placenta and fetalmembranes (Fig. 1b and Extended Data Fig. 1a–c) in 60% of the chimaeric embryos (Fig. 1c).」ですね。
STAP cells contributed not only to the embryo but also to the placenta and fetalmembranes (Fig. 1b and Extended Data Fig. 1a–c) in 60% of the chimaeric embryos (Fig. 1c).と
書いたのは笹井さんよ。not only to the embryo but also to the placenta and fetalmembranesと小保方さんの原稿にあったとしてもand fetal membranes と続いたときに気づくでしょ。
彼は気づかなかったのよ。なぜ？
>These results support the view that the RNA-seq data contained transcripts from two major cell populations, with approximately 90% B6 cells having an ESC-like expression pattern and approximately 10% CD1-like cells having a TSC-like pattern. Therefore, the claim in the Obokata et al. paper that FI-SCs contribute to the placenta might have been based in error on contamination by TSCs, which are known to be organ stem cells (Tanaka et al. 1998).
１１次元のところに答えがあるようですね。Ｐｕｂｐｅｅｒの記事の紹介があるんで
そこに入り込んでかなり下の方まで読み進むと、専門家らしい人の意見があって
"Mouse phenotypes: a Handbook of mutation analysis"を参照しろと
あって、ＥＳで胎膜が光ると書いてある。
The authors claim that "Surprisingly, injected STAP cells contributed not only to the embryo but also to the placenta and fetal membranes (Fig. 1b and Extended Data Fig. 1a??"c) in 60% of the chimaeric embryos (Fig. 1c)." and they show the analysis of chimaeric embryos with placentas at E12.5. I'm sorry to say but that is normal in E12.5 chimaeric embryos produced by injection of wt ES cells into blastocyst, since the placental FETAL blood vessels and yolk sac mesoderm cells are derived from the ES-cell derived epiblast.
