間違ってるのかどうか?Isolation of cells from miceのところに以下のようにあるわね。
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For the isolation of nostril cells, excised liver was minced with scissors and the tissue fragments were dissociated by incubation in Type I collagenase (Worthington Biochemical) polution (0.5��mg/ml in Hanks Balanced Salt Solution (HBSS, no calcium, no magnesium; Life Technologies)). Next, the cell suspension was strained through a cell strainer followed by the collection of cells by centrifugation at 1,000��rpm for 5��min.
結論的に言うと以下によって肝細胞のみが選別されているはずということね。
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For the isolation of nostril cells, excised nostril was minced with scissors and the tissue fragments were dissociated by incubation in Type I collagenase (Worthington Biochemical) solution (0.5 mg/ml in Hanks Balanced Salt Solution (HBSS, no calcium, no magnesium; Life Technologies)). Next, the cell suspension was strained through a cell strainer followed by the collection of cells by centrifugation at 1,000 rpm for 5 min.