㉖本論に戻ってDBの＜C57BL/6x129/Sv＞としたのは、小保方さんなのか、ラボ仲間の手伝いをしてくれた人なのか、
登録時の理研の事務員なのかを明らかにしろ。小保方さんは親マウスの雌雄をちゃんとルールに従って記載している。
②129/Sv x B6-CAG-GFP
③B6-CAG-GFP x 129/Sv
は書き分けられている。
論文にはSTAP幹細胞転換培養のところで、使われたストレーンのＦ1は②だと明確に書かれている。
まずは論文は以下だ。
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STAP stem-cell conversion culture
For establishment of STAP stem-cell lines, STAP cell clusters were transferred to ACTH-containing medium36 on MEF feeder cells (several clusters, up to a dozen clusters, per well of 96-well plates). Four to seven days later, the cells were subjected to the first passage using a conventional trypsin method, and suspended cells were plated in ES maintain medium containing 20% FBS. Subsequent passaging was performed at a split ratio of 1:10 every second day before they reached subconfluency. We tested the following three different genetic backgrounds of mice for STAP stem-cell establishment from STAP cell clusters, and observed reproducible data of establishment: C57BL/6 carrying Oct4-gfp (29 of 29), 129/Sv carrying Rosa26-gfp (2 of 2) and 129/Sv × C57BL/6 carrying cag-gfp (12 of 16). STAP stem cells with all these genetic backgrounds showed chimaera-forming activity.
