まず③に関してはBCA報告はこのGRASに提出された時のマウス背景に関する記述内容を
何も示していないね。
>>
To gain sufficient sequen-cing coverage, we re-sequenced the genomic DNA prepared from the STAP cell lysate used for ChIP-seq (Extended Data Fig. 1a). We con-firmed that this STAP cell sample shared all the genomic character-istics described above for 129B6F1 ES1 (Extended Data Fig. 2c),indicating that the STAP cell sample used for ChIP-seq was derivedfrom 129B6F1 ES1 cells.
キメラのところというより、幹細胞のところでしょ。ここでしょ。
>>
STAP stem-cell conversion culture
For establishment of STAP stem-cell lines, STAP cell clusters were transferred to ACTH-containing medium36 on MEF feeder cells (several clusters, up to a dozen clusters, per well of 96-well plates). Four to seven days later, the cells were subjected to the first passage using a conventional trypsin method, and suspended cells were plated in ES maintain medium containing 20% FBS. Subsequent passaging was performed at a split ratio of 1:10 every second day before they reached subconfluency. We tested the following three different genetic backgrounds of mice for STAP stem-cell establishment from STAP cell clusters, and observed reproducible data of establishment: C57BL/6 carrying Oct4-gfp (29 of 29), 129/Sv carrying Rosa26-gfp (2 of 2) and 129/Sv × C57BL/6 carrying cag-gfp (12 of 16). STAP stem cells with all these genetic backgrounds showed chimaera-forming activity.
