Brief Report
Successful Serial Recloning in the Mouse over Multiple Generations
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SayakaWakayama1TakashiKohda2HarukoObokata13MikikoTokoro14ChongLi15YukariTerashita16EijiMizutani17Van ThuanNguyen18SatoshiKishigami19FumitoshiIshino2TeruhikoWakayama17
この論文で使われている連続クローンのドナーマウスの背景は BD129F1だね。
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We used four BD129F1 female mice (BDF1 × 129/Sv) as nuclear donors, and the first generation of cloned mice (hereafter termed G1) was produced from the cumulus cell nuclei of those donors. These mice were produced in our laboratory with three-way crosses between C56/BL6, DBA/2, and 129/Sv strains.
特許でも、同じ表が使われていますがラインは入っていません。
Natureの査読で
「Table 1 reports offspring, but some of the chimaeras are analysed as foetuses. Please discriminate. 」
と、指摘されて入れたラインでは？
我々が言ってるのは無論Extendes Data Figure 7 のことだね。ネイチャーのレフェリーの
#2さんが言ってるのは、3月に提出された現行の図表のTable 1のことだけど、最終稿では無論
改定されてしまっているから元がどうであったかは分からないね。
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Important Data on chimaera production are presented in Supplementary Tables, but these also are not clear or not comprehensive.Table 1 reports offspring, but some of the chimaeras are analysed as foetuses. Please discriminate.
何か随分分かってきたな。僕はDBA/2のF1は2011年に何かしたのかと思ってた。
ただ、この最終稿の表も矛盾が多いからね。
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**The contribution of STAP cells into each chimaera was scored as high (>50% of the coat colour of GFP expression).
本文での答えは以下よね。
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Because the number of CD45+ cells from a neonatal spleen was small, we mixed spleen cells from male and female mice for STAP cell conversion. To make germline transmission more efficient, we intercrossed chimaeras in some experiments.
あなたたちがイライラするとすぐ人の悪口を言いたくなるように#2さんもきっと
いらいらしたのよね。
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The Data in the table can not be fully interpreted without information on the strain, genotype and gender of the STAP cells.
この結果から
丹羽Protocol
(iv) Cells from mice older than one week showed very poor reprogramming efficiency under the current protocol. Cells from male animals showed higher efficiency than those from female.
