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Figure 12 Mesenchymal lineage differentiation.
Dissociated spheres were plated into serum-containing medium and cultured for 14-21 days. Plated cells differentiated into mesenchymal lineage cells even plated cells were from spheres derived from endoderm or ectoderm tissues.
Marrow spheres differentiated into condrocytes (A), adipocytes (B) and osteocytes (C). Pnemospheres differentiated into condrocytes (D), adipocytes (E) and osteocytes (F). Spinalspheres differentiated into condrocytes (G) and adipocytes (H).
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Figure 13 Teratoma forming assay
10<to the power of 7> bone marrow cells and ES cells were injected subcutaneously into immunedificienl mice.
After 6 weeks of implantation, cell masses were harvested.
Figure 14 Teratoma like mass from bone marrow spheres contained nerve expressing betalll-tubuline (left)(ectoderm), muscle expressing desmin (middle)(mesoderm) and duct like structure expressing AFP (right)(endoderm).
1. Berrier, A. L.; Yamada, K. M. Cell-matrix adhesion. J. Cell. Physiol.213:565□573: 2007.
2. Braam, S. R.; Zeinstra, L.i Litjens, S.; Ward-van Oostwaard, D.; van den Brink, S.; van Laake, L.; Lebrin, F.; Kats, P.; Hochstenbach, R.; Passier, R.; Sonnenberg, A.; Mummery, C. L. Recombinant vitronectin is a functionally defined substrate that supports human embryonic stem cell self-renewal via alphavbetaS integrin. Stem Cells 26-2257□2265: 2008.
3. Brakebusch, C.; Fassler, R. The integrin-actin connection, an eternal love affair. EMBO J. 22:2324□2333: 2003.
(英文)
4. Busser, B. W.; Bulyk, M. L.; Michelson A. M. Toward a systems-level understanding of developmental regulatory networks. Curr. Opin. Genet. Dev. 18:521□529: 2008.
5. Casella, J. F.; Flanagan, M. D.; Lin, S. Cytochalasin D inhibits actin polymerization and induces depolymerization of actin filaments formed during platelet shape change. Nature 293-302□305: 1981.
6. Ceradini, D. J.; Kulkarni, A. R.l Callaghan, M. J.; Tepper, O. M.I Bastidas, N.;Kleinman, M. E.; Capla, J. M.; Galiano, R. D.; Levine, J. P.I Gurtner, G. C. Progenitor cell trafficking is regulated by hypoxic gradients through HIF-1 induction of SDF-1. Nat. Med. 10-858□864: 2004.
7. Chelberg, M. K.I Tsilibary, E. C.l Hauser, A. R.l McCarthy, J. B. Type IV coUagen-mediated melanoma cell adhesion and migration: involvement of multiple, distinct domains of the collagen molecule. Cancer Res. 49:4796□4802; 1989.
8. Chiou, S. H.; Kao, C. L.; Peng, C. H.; Chen, S. J.; Tarng, Y. W.; Ku, H. H.; Chen, Y. C.; Shyr, Y. M.; Liu, R. S.; Hsu, C. J.; Yang, D. M.; Hsul W. M.; Kuo, C. D.; Lee, C. H. A novel in vitro retinal differentiation model by co'culturing adult human bone marrow stem cells with retinal pigmented epithelium cells. Biochem. Biophys. Res. Commun. 326:578□585; 2005.
4. Busser, B. W.; Bulyk, M. L.; Michelson A. M. 発達規制ネットワークのシステムレベルの理解に向けて。 Curr. Opin. Genet. Dev. 18:521□529: 2008.
5. Casella, J. F.; Flanagan, M. D.; Lin, S. サイトカラシンDはアクチン重合を阻害し、血小板形状変化の間に形成されるアクチンフィラメントの解重合を誘導する。 Nature 293-302□305: 1981.
6. Ceradini, D. J.l Kulkarni, A. R.l Callaghan, M. J.I Tepper, O. M.I Bastidas, N.;Kleinman, M. E.; Capla, J. M.I Galiano, R. D.I Levine, J. P.I Gurtner, G. C. 前駆細胞の輸送は、SDF-1のHIF-1誘導を介して、低酸素勾配によって調節される。 Nat. Med. 10-858□864: 2004.
7. Chelberg, M. K.I Tsilibary, E. C.l Hauser, A. R.l McCarthy, J. B. IV型コラーゲン媒介黒色腫細胞の接着および遊走：コラーゲン分子の複数別個領域の関与。 Cancer Res. 49:4796□4802; 1989.
8. Chiou, S. H.; Kao, C. L.; Peng, C. H.; Chen, S. J.; Tarng, Y. W.; Ku, H. H.; Chen, Y. C.; Shyr, Y. M.; Liu, R. S.; Hsu, C. J.; Yang, D. M.; Hsul W. M.; Kuo, C. D.; Lee, C. H. 成人ヒト骨髄幹細胞を網膜色素上皮細胞と共培養することによる新規試験管内網膜分化モデル Biochem. Biophys. Res. Commun. 326:578□585; 2005.
(英文)
9. Choi, J. S.; Yang, H. J.; Kim, B. S.; Kim, J. D.; Kim, J. Y.; Yoo, B.; Park, K.; Lee, H. Y.; Cho, Y. W. Human extracellular matrix (ECM) powders for injectable cell delivery and adipose tissue engineering. J. Control. Release 139:2□7; 2009.
10. Cooper, H. M.; Tamura, R. N.; Quaranta, V. The major laminin receptor of mouse embryonic stem cells is a novel isoform of the alpha 6 beta 1 integrin. J. CellBiol. 115:843□850; 1991.
11. Czirok, A.; Zamir, E. A.; Filla, M. B.; Little, C. D.; Rongisli, B. J. Extracellular matrix macroassembly dynamics in early vertebrate embryos. Curr. Top. Dev. Biol. 73:237□258; 2006.
12. Decline, F.; Rousselle, P. Keratinocyte migration requires alpha2beta1 integrin-mediated interaction with the laminin 5 gamma2 chain. J. Cell Sci. 114:811□823; 2001.
13. Desban, N.; Lissitzky, J. C.; Rousselle, P.; Duband, J. L. alpha1beta1-integrin engagement to distinct laminin-1 domains orchestrates spreading, migration and survival of neural crest cells through independent signaling pathways. J. Cell Sci. 119:3206□3218; 2006.
9. Choi, J. S.; Yang, H. J.; Kim, B. S.; Kim, J. D.; Kim, J. Y.; Yoo, B.; Park, K.; Lee, H. Y.; Cho, Y. W. 注射による細胞送達および脂肪組織工学のためのヒト細胞外マトリックス（ECM）粉末。 J. Control. Release 139:2□7; 2009.
10. Cooper, H. M.; Tamura, R. N.; Quaranta, V. マウス胚性幹細胞の主要なラミニン受容体は、α6β1インテグリンの新規アイソフォームである。 J. CellBiol. 115:843□850; 1991.
11. Czirok, A.; Zamir, E. A.; Filla, M. B.; Little, C. D.; Rongisli, B. J. 初期の脊椎動物の胚における細胞外マトリックスのマクロダイナミクス Curr. Top. Dev. Biol. 73:237□258; 2006.
12. Decline, F.; Rousselle, P. ケラチノサイトの移動は、ラミニン5γ2鎖とのα2β1インテグリン媒介相互作用を必要とする。 J. Cell Sci. 114:811□823; 2001.
13. Desban, N.; Lissitzky, J. C.; Rousselle, P.; Duband, J. L. 異なるラミニン-1ドメインへのα1β1インテグリンの関与は、独立したシグナル伝達経路を介した神経堤細胞の広がり、遊走および生存を調整する。 J. Cell Sci. 119:3206□3218; 2006.
(英文)
14. Goto, M.; Sumiyoshi, H.; Sakai, T.J Fassler, R.! Ohashi, S.j Adachi, E.. Yoshioka, H.; Fujiwara, S. Ehmination ofepiplakin by gene targeting results in acceleration ofkeratinocyte migration in mice. Mol. Cell. Biol. 26:548□558; 2006
15. Hayashi, Y.; Furue, M. K; Okamoto, T.: Ohnuma, K.; Myoishi, Y.: Fukuhara, Y.: Abe, T.; Sato, J. D.; Hata, R.; Asasliima, M. Integrins regulate mouse embryonic stem cell self-renewal. Stem Cells 25-3005□3015; 2007.
16. Hehlgans, S.; Haase, M.; Cordes, N. Signalling via integrins: implications for cell survival and anticancer strategies. Biochim. Biophys. Acta 1775:163□180; 2007
17. Hynes, R. O. Integrins: bidirectional, allosteric signaling machines. Cell 110:673□687; 2002.
18. Khoshnoodi, J.; Pedchenko, V.; Hudson, B. G. Mammalian collagen IV. Microsc. Res. Tech. 71:357□370; 2008.
(英文)
19. Kim, Y. S.; Park, H. J.; Hong, M. H.; Kang, P. M.; Morgan, J. P.; Jeong, M. H.; Cho, J. G.: Park, J. C.; Ahn, Y. TNF-alpha enhances engraftment of mesenchymal stem cells into infarcted myocardium. Front. Biosci. 14:2845□2856: 2009.
20. Kjtaori, T.; Ito, H.; Schwarz, E. M.: Tsutsumi, R.; Yoshitomi, H.; Oishi, S.;Nakano, M.; Fujii, N.; Nagasawa, T.: Nakamura, T. Stromal cell-derived factor 1/CXCR4 signaling is critical for the recruitment of mesenchymal stem cells to the fracture site during skeletal repair in a mouse model. Arthritis Rheum. 60:813□823; 2009.
21. Kofidis, T.; de Bruin, J. L.; Yamane, T.: Balsam, L. B.; Lebl, D. R.; Swijnenburg, R. J. :Tanaka, M.; Weissman, I. L.; Robbins, R. C. Insulin-like growth factor promotes engraftment, differentiation, and functional improvement after transfer of embryonic stem cells for myocardial restoration. Stem Cells 22;1239□1245; 2004.
22. Kofidis, T.: de Bruin, J. L.: Yamane, T.; Tanaka, M.; Lebl, D. R.; Swijnenburg, R. J.; Weissman, L L.; Robbins, R. C. Stimulation of paracrine pathways with growth factors enhances embryonic stem cell engraftment and host-specific differentiation in the heart after ischemic myocardial injury. Circulation 111:2486□2493: 2005.
23. Laflamme, M. A.: Chen, K. Y.; Naumova, A. V.; Muskheli, V.; Fugate, J. A.; Dupras, S. K.; Reinecke, H.; Xu, C.; Hassanipour, M.; Pohce, S.; O'Sullivan, C.; Collins, L.; Chen, Y.; Minami, E.; Gill, E. A.; Ueno, S.; Yuan, C.; Gold, J.; Murry, C. E. Cardiomyocytes derived from human embryonic stem cells in pro-survival factors enhance function of infarcted rat hearts. Nat. Biotechnol. 25:1015□1024: 2007.
19. Kim, Y. S.; Park, H. J.; Hong, M. H.; Kang, P. M.; Morgan, J. P.; Jeong, M. H.; Cho, J. G.: Park, J. C.; Ahn, Y.TNF-アルファは、間葉系幹細胞の梗塞心筋への移植を促進する。 Front. Biosci. 14:2845□2856: 2009.
20. Kjtaori, T.; Ito, H.; Schwarz, E. M.: Tsutsumi, R.; Yoshitomi, H.; Oishi, S.;Nakano, M.; Fujii, N.; Nagasawa, T.: Nakamura, T. 間質細胞由来因子1 / CXCR4シグナル伝達は、マウスモデルにおける骨格修復の間に骨折部位への間葉系幹細胞の動員にとって重要である。 Arthritis Rheum. 60:813□823; 2009.
21. Kofidis, T.; de Bruin, J. L.; Yamane, T.: Balsam, L. B.; Lebl, D. R.; Swijnenburg, R. J. :Tanaka, M.; Weissman, I. L.; Robbins, R. C. インスリン様増殖因子は、心筋再生のための胚性幹細胞移植後の生着、分化および機能改善を促進する。 Stem Cells 22;1239□1245; 2004.
22. Kofidis, T.: de Bruin, J. L.: Yamane, T.; Tanaka, M.; Lebl, D. R.; Swijnenburg, R. J.; Weissman, L L.; Robbins, R. C. 成長因子によるパラクリン経路の刺激は、虚血性心筋損傷後の心臓における胚性幹細胞の生着および宿主特異的分化を増強する。 Circulation 111:2486□2493: 2005.
23. Laflamme, M. A.: Chen, K. Y.; Naumova, A. V.; Muskheli, V.; Fugate, J. A.; Dupras, S. K.; Reinecke, H.; Xu, C.; Hassanipour, M.; Pohce, S.; O'Sullivan, C.; Collins, L.; Chen, Y.; Minami, E.; Gill, E. A.; Ueno, S.; Yuan, C.; Gold, J.; Murry, C. E. 生存促進因子におけるヒト胚性幹細胞由来の心筋細胞は、梗塞したラット心臓の機能を高める。Nat. Biotechnol. 25:1015□1024: 2007.
(英文)
24. Lee, J. M.; Dedhar, S.; Kalluri, R.I Thompson, E. W. The epithelial-mesenchymal transition- new insights in signaling, development, and disease. J. Cell Biol. 172:973□981; 2006.
25. Lee, K. H.; Chuang, C. K; Wang, H. W.: Stone, L.; Chen, C. H.; Tu, C. F. An alternative simple method for mass production of chimeric embryos by coculturing deヌded embryos and embryonic stem cells in Eppendorf vials. Theriogenology 67;228□237; 2007.
27. Li, K.; Chuen, C. K.; Lee, S. M.; Law, P.; Fok, T. F.; Ng, P. C.; Li, C. K; Wong, D.: Merzouk, A.; Salad, H.; Gu, G. J.; Yuen, P. M. Small peptide アnalogue of SDF-lalpha supports survival of cord blood CD34+ cells in synergy with other cytokines and enhances their ex vivo expansion and engraftment into nonobese diabetic/severe combined immunodeficient mice. Stem Cells 24:55□64: 2006.
28. Li, S.; Tanaka, H.; Wang, H. H.; Yoshiyama, S.; Kumagai, H.; Nakamura, A.; Brown, D. L.; Thatcher, S. E..;Wright, G. L.; Kohama, K. Intracellular signal transduction for migration and actin remodeling in vascular smooth muscle cells after sphingosylphosphorylcholine stimulation. Am. J. Physiol. Heart Circ. Physiol. 291:H1262□1272; 2006.
24. Lee, J. M.; Dedhar, S.; Kalluri, R.I Thompson, E. W. 上皮間葉移行 - シグナル伝達、発生および疾患における新しい洞察。 J. Cell Biol. 172:973□981; 2006.
25. Lee, K. H.; Chuang, C. K; Wang, H. W.: Stone, L.; Chen, C. H.; Tu, C. F. 裸胚と胚性幹細胞をエッペンドルフバイアルに共培養することにより、キメラ胚の大量生産のための簡単な代替方法。Theriogenology 67;228□237; 2007.
26. Leitinger, B.; Hohenester, E. 哺乳類のコラーゲン受容体。 26:146-155; 2007.
27. Li, K.; Chuen, C. K.; Lee, S. M.; Law, P.; Fok, T. F.; Ng, P. C.; Li, C. K; Wong, D.: Merzouk, A.; Salad, H.; Gu, G. J.; Yuen, P. M. SDF-1αの小ペプチド類似体は、他のサイトカインとの相乗作用で臍帯血CD34 陽性細胞の生存を助け、それらの体外での増殖および非肥満糖尿病/重症複合免疫不全マウスへの移植を補強する。 Stem Cells 24:55□64: 2006.
28. Li, S.; Tanaka, H.; Wang, H. H.; Yoshiyama, S.; Kumagai, H.; Nakamura, A.; Brown, D. L.; Thatcher, S. E..;Wright, G. L.; Kohama, K. スフィンゴシルホスホリルコリン刺激後の血管平滑筋細胞における遊走およびアクチン再構築のための細胞内シグナル伝達。 Am. J. Physiol. Heart Circ. Physiol. 291:H1262□1272; 2006.
(英文)
29. Lock, J. G.; Wehrle-Haller, B.i Stromblad, S. Cell-matrix adhesion complexes: master control machinery of cell migration. Semin. Cancer Biol. 18:65□76; 2008
30. Matsuda, R.: Yoshikawa, M.; Kimura, H.; Ouji, Y.;Nakase, H.; Nishimura, F.; Nonaka, J.; Toriumi, H.; Yamada, S.; Nishiofuku, M.; Moriya, K.; Ishizaka, S.; Nakamura, M.; Sakaki, T. Cotransplantation of mouse embryonic stem cells and bone marrow stromal cells following spinal cord injury suppresses tumor development. Cell Transplant. 18:39□54; 2009.
31. Mooney, D. J.; Vandenburgh, H. Cell delivery mechanisms for tissue repair. Cell Stem Cell 2:205□213; 2008.
32. Muny, C. E.; Keller, G. Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development. Cell 132:661□680; 2008
33. Nishikawa, S.; Jakt, L. M.; Era, T. Embryonic stem-cell culture as a tool for developmental cell biology. Nat. Rev. Mol. Cell Biol. 8-502□507; 2007.
(英文)
34. Pan, G.; Thomson, J. A. Nanog and transcriptional networks in embryonic stem cell pluripotency. Cell Res. 17:42□49: 2007.
35. Parameswaran, K.; Radford, K.; Zuo, J.; Janssen, L. J.; O'Byrne, P. M.; Cox, P. G. Extracellular matrix regulates human airway smooth muscle cell migration. Eur. Respir. J. 24;545□55l; 2004.
36. Passier, R.; van Laake, L. W.; Mummery, C. L. Stem-cell-based therapy and lessons from the heart. Nature 453:322□329; 2008.
37. Perris, R.; Syfrig, J.; Paulsson, M.; Bronner-Fraser, M. Molecular mechanisms of neural crest cell attachment and migration on types 1 and IV collagen. J. Cell Sci. 106(Pt4):1357□1368; 1993.
38. Poschl, E.; Schlotzer-Schrehardt, U.; Brachvogel, B.; Saito, K.; Ninomiya, Y.: Mayer, U. Collagen IV is essential for basement membrane stability but dispensable for initiation of its assembly during early development.
