解散だな。賛成。不要だ。
Hereafter, we refer to the fate conversion from somatic cells into pluripotent cells by strong external stimuli such as low pH as ‘stimulus-triggered acquisition of pluripotency’ (STAP) and the resultant cells as STAP cells. Under their establishment conditions, these STAP cells were rarely proliferative (Extended Data Figs 2d and 5a, b). Comparative genomic hybridization array アナリシス of STAP cells indicated no major global changes in chromosome number (Extended Data Fig. 5c).
つぎはＥＳ比較だ。
STAP cells compared to ES cells
STAP cells, unlike mouse ES cells, showed a limited capacity for self-renewal in the LIF-containing medium and did not efficiently form colonies in dissociation culture (Fig. 2f, g), even in the presence of the ROCK inhibitor Y-27632, which suppresses dissociation-induced apoptosis (Fig. 2h). Also, even under high-density culture conditions after partial dissociation (Fig. 2i), STAP cell numbers started to decline substantially after two passages. Furthermore, expression of the ES cell marker protein Esrrβ was low in STAP cells (Extended Data Fig. 5d, e). In general, female ES cells do not show X-chromosomal inactivation and contain no H3K27me3-dense foci (indicative of inactivated X chromosomes), unlike female CD45+ cells and EpiSCs. In contrast, H3K27me3-dense foci were found in ~40% of female STAP cells strongly positive for Oct4-GFP (Extended Data Fig. 5f, g).
続き
STAP cells were also dissimilar to mouse EpiSCs, another category of pluripotent stem cell, and were positive for Klf4 and negative for the epithelial tight junction markers claudin 7 and ZO-1 (Extended Data Fig. 5d, e).
ドンドン行こう。
STAP cells from other tissue sources
We next performed similar conversion experiments with somatic cells collected from brain, skin, muscle, fat, bone marrow, lung and liver tissues of 1-week-old Oct4-gfp mice. Although conversion efficacy varied, the low-pH-triggered generation of Oct4-GFP+ cells was observed in day 7 culture of all tissues
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examined (Fig. 3a and Extended Data Fig. 6a–c), including mesenchymal cells of adipose tissues (Fig. 3a–c) and neonatal cardiac cells that were negatively sorted for CD45 by FACS (Fig. 3d–g; see Extended Data Fig. 6d for suppression of cardiac genes such as Nkx2-5 and cardiac actin).
次がいよいよキメラよね。ひどいよ。
We next performed a blastocyst injection assay with STAP cells that were generated from CD45+ cells of neonatal mice constitutively expressing GFP (this C57BL/6 line with cag-gfp transgenes is referred to hereafter as B6GFP). We injected STAP cell clusters en bloc that were manually cut into small pieces using a microknife (Fig. 4a). A high-to-moderate contribution of GFP-expressing cells was seen in the chimaeric embryos (Fig. 4b and Extended Data Fig. 7a). These chimaeric mice were born at a substantial rate and all developed normally (Fig. 4c and Extended Data Fig. 7b).
Ｂ6ＧＦＰという言い方はこの論文で小保方さんがそう呼んでるということだからね。
this C57BL/6 line with cag-gfp transgenes is referred to hereafter as B6GFPというんだから
若山さんの言葉なら（129ＳｖＸＢ6）Ｆ1 ｃａｇ-ｇｆｐ しかもホモなんだよね。
それを小保方さんはＣ57ＢＬ/6のＣＡＧ-ＧＦＰだと思っていてかつそれをここでは
Ｂ6ＧＦＰと呼ぶと言ってるわけだけど、マウス系統は呼び方決まってるんでしょ。
こんなに勝手に呼び方自分流にしてはいけないでしょ。
