Article Figure 6とExtended Data Figure 8のリジェンドは以下だ。
(Article Figure 6)
a, Growth of STAP stem cells carrying Oct4-gfp. Scale bar, 50 μm. b, Dissociation culture of STAP stem cells to form colonies. Scale bar, 100 μm. c, Robust growth of STAP stem cells in maintenance culture. Similar results were obtained with eight independent lines. In contrast, parental STAP cells decreased in number quickly. d, Immunostaining of STAP stem cells for pluripotency markers (red). Scale bar, 50 μm. e, qPCR analysis of pluripotency marker gene expression. f–h, In vitro differentiation assays into three-germ-layer derivatives.
f, Ectoderm: Rx+/Pax6+ (retinal epithelium; 83%, n = 6). g, Mesoderm: troponin-T+ (cardiac muscle; 50%, n = 6). h, Endoderm: Sox17+/E-cadherin+ (endodermal progenitors; 67%, n = 6). Scale bar, 50 μm. i, Teratoma formation assays. Formation of keratinized epidermis (ectoderm; left), cartilage (mesoderm; middle) and bronchial-like epithelium (endoderm; right) is shown. Scale bar, 100 μm. j, Blastocyst injection assays. These pictures of live animals were taken serially (asterisk indicates the same chimaeric pup). k, l, Tetraploid complementation assay. ‘All-GFP+’ pups were born (k) and germline transmission was observed (l).
