(続き)
STAP stem cells expressed protein and RNA markers for pluripotent cells (Fig. 5d, e), showed low DNA methylation levels at the Oct4 and Nanog loci (Extended Data Fig. 8d), and had a nuclear fine structure similar to that of ES cells (Extended Data Fig. 8e; few electron-dense areas corresponding to heterochromatin). In differentiation culture25, 26, 27, STAP stem cells generated ectodermal, mesodermal and endodermal derivatives in vitro (Fig. 5f–h and Extended Data Fig. 8f, g), including beating cardiac muscles (Supplementary Video 4), and formed teratomas in vivo (Fig.5i and Extended Data Fig. 8h; no teratocarcinomas, n = 40). After blastocyst injection, STAP stem cells efficiently contributed to chimaeric mice (Fig. 5j), in which germline transmission was seen (Extended Data Fig. 8i). Even in tetraploid complementation assays, injected STAP stem cells could generate mice capable of growing to adults and producing offspring (Fig. 5k, l; in all eight independent lines, Extended Data Fig. 8j).
