(英文)
Contents
1. Background
1.1 General Introduction - 1
• Importance of stem cells
• Unique properties of all stem cells
1.2 Adult stem cell - 7
• History of Adult stem cell research
• Function of adult stem cells
1.3 Research methods for identifying adult stem cells - 10
• Normal differentiation pathways of adult stem cells.
• Transdifferentiation.
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1.4 Pluripotent stem cells - 14
• The similarities and differences between embryonic and adult stem cells
• Embryonic stem cells
A. Establish<→Estalishment> of embryonic stem cells grown in the laboratory
B. Tests for identifying embryonic stem cells
• Induced pluripotent stem cells
1.5 Possibility of existence of adult pluripotent stem cells - 20
• Spore-like stem cells
1.6 Hypothesis of this research - 23
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4. Isolation of small cells from TISSUES DERIVED FROM ALL THREE GERM LAYERS
4.1 Introduction - 63
4.2 Experimental<→Experiment> - 63
4.2.1 Isolation of small cells from various tissues - 64
4.2.2 Characterization of isolated small cells - 64
4.2.3 Differentiation potential of sphere forming small cells - 64
4.3 Results - 65
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4.3.1 Sphere formation from tissues derived from representative of the three germ layers - 65
4.3.2 Immature gene expression of spheres derived from tissues representative of the three germ layers -66
4.3.3 Differentiation potential of Cells derived from tissues representative of the three germ layers-67
4.3.4 Differentiation potential of cells in vivo - 68
4.4 Summary of section 4 - 69
4.5 Discussion - 69
4.6 References - 76