2008年論文は特許と関連してる論文ね。
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ES Cell Lines
The D3 ES cell line was originally established by Doetschman et al. [12], and its GFP transgenic cell line (129SV(D3)-Tg(ACTB-EGFP)CZ-001-FM260Osb), which was established using pCAG-EGFP [10, 11], was kindly provided by Dr. Masaru Okabe (Osaka University, Osaka, Japan). The E14 ES cell line [7] was derived from the inbred mouse strain 129/Ola in 1985 by Dr. Martin Hooper in Edinburgh (Scotland) and was obtained through Dr. Peter Mombaerts (Rockefeller University). The 129B6F1G1 [13] and BDmt2 [14] are nuclear transfer-derived ES (ntES) cell lines [15] previously established in our laboratory using Sertoli cells of 129B6F1 background with GFP and tail-tip cells of a male BDF1 mouse as donor for nuclear transfer, respectively. Male 129B6F1 mice expressing GFP were generated by mating female 129/Sv mice with male C57BL/6 GFP transgenic mice (double-transgenic mouse line [16] constructed using pCAG-EGFP [10, 11] and Acr3-EGFP [17]).
この４行目にThe 129B6F1G1 [13] とあって13の注に2005年論文が指定されている。
そしてその後にSertoli cells of 129B6F1 background with GFPとあるね。そこまで読んでいるのに
次の文章にMale 129B6F1 mice expressing GFP were generated by mating female 129/Sv mice
with male C57BL/6 GFP transgenic mice (double-transgenic mouse line [16] constructed
using pCAG-EGFP [10, 11] and Acr3-EGFP [17]).と続いているのを読み過ごしている。
2005年論文に遡らなくても親の雌雄は再確認されているね。
2005年論文も多分流し読みされたわね。
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Mice
Immature male B6C3F1, adult female ICR, and adult female BDF1 mice were purchased from Shizuoka Laboratory Animal Center (Hamamatsu, Japan). Female 129/Sv-ter and male ICR n ude mice were purchased from CLEA Japan, Inc., and Charles River Japan, Inc., respectively. The green fluorescent protein (GFP) transgenic mice carrying both the acrosin/eGFP (Acr3-EGFP) [34] and pCX-eGFP [35] transgenes (C57BL/6TgN(acro/act-EGFP)OsbC3-N01-FJ002 [36]) were kindly provided by Dr. M. Okabe (Osaka University, Osaka, Japan; all strain designations are those of the original studies). To generate 129B6F1 mice carrying the GFP transgene, female 129/Sv-ter mice were mated with male C57BL/6 GFP transgenic mice, and the offspring of these matings, which were hemizygous for the GFP transgene, were used as donors for nuclear transplantation. All animal experiments conformed to the Guide for the Care and Use of Laboratory Animals and were approved by the Institutional Committee of Laboratory Animal Experimentation of the RIKEN Kobe Institute.
５行目にあるね。129Terとより詳しく書かれている。
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To generate 129B6F1 mice carrying the GFP transgene, female 129/Sv-ter mice were mated with male C57BL/6 GFP transgenic mice, and the offspring of these matings, which were hemizygous for the GFP transgene, were used as donors for nuclear transplantation.
PGAというのはティシュー論文にある PolyGlycolic Acid fibersの略ね。
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This solution was seeded onto a sheet 3x3x1mm, composed of a nonwoven mesh of polyglycolic acid fibers, 200mm in diameter, and implanted subcutaneously into the dorsal flanks of a 4-week-old NOD/SCID mice (Jackson Laboratory).
アスペルガーらしいのはいるなぁ。アノ何とかとつや何とか。典型的だなぁ。カルト教主だったら大成したのに。標本的としてため息を原発巣とするMetastatic tumor 君達のコメントは凄すぎるなあ。Kind is called a friend だよな。 但しアスペルガー犯罪の実例は多々あるが、社会的な犯罪を起こさない生育環境で育った成功例だよな。スポーツでは大リーグ狂、サッカー狂、ジャンプ狂。疫学的知見では子供が作れない傾向にあるとなっているらしいが例外もあるらしい。アスペルガーの男は寂聴さんの言葉を使わせてもらえば、奥さんは不感症と言う事になるのかな。不感症と子宮筋腫の関連性の報告が何処かにあったな。
はいな。
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6weeks +PGA 12/27 移植 Haruko
PGAというのはティシュー論文にある PolyGlycolic Acid fibersの略ね。
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This solution was seeded onto a sheet 3x3x1mm, composed of a nonwoven mesh of polyglycolic acid fibers, 200mm in diameter, and implanted subcutaneously into the dorsal flanks of a 4-week-old NOD/SCID mice (Jackson Laboratory).
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①12/10(土曜日）から12/18(日曜日)の9日間 〇無し
②12/24(土曜日)から1/22(日曜日)の３０日間 〇無し
③2012年1月度23日月曜から平日全て「まる」
④2月度、23日以外、平日全「まる」
普通に考えればそうだね。特に<10の5乗ずつ移植>という記述はティシュー論文の
作り方と違うね。
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Materials and Methods
In vivo differentiation assay
Sphere cultures from representative tissues were washed twice with Hank’s balanced salt solution (Gibco). Under a microscope, 2000 spheres, each containing *1000 cells, were collected using a glass pipette and placed in a 50mL tube. Hank’s balanced salt solution (20mL) was added to each tube and subsequently centrifuged at 800rpm for 3min. The supernatant was discarded and the pellet was resuspended in 50mL of DMEM with 10% fetal bovine serum. This solution was seeded onto a sheet 3x3x1mm, composed of a nonwoven mesh of polyglycolic acid fibers, 200mm in diameter, and implanted subcutaneously into the dorsal flanks of a 4-week-old NOD/SCID mice (Jackson Laboratory).
