2008年論文は特許と関連してる論文ね。
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ES Cell Lines
The D3 ES cell line was originally established by Doetschman et al. [12], and its GFP transgenic cell line (129SV(D3)-Tg(ACTB-EGFP)CZ-001-FM260Osb), which was established using pCAG-EGFP [10, 11], was kindly provided by Dr. Masaru Okabe (Osaka University, Osaka, Japan). The E14 ES cell line [7] was derived from the inbred mouse strain 129/Ola in 1985 by Dr. Martin Hooper in Edinburgh (Scotland) and was obtained through Dr. Peter Mombaerts (Rockefeller University). The 129B6F1G1 [13] and BDmt2 [14] are nuclear transfer-derived ES (ntES) cell lines [15] previously established in our laboratory using Sertoli cells of 129B6F1 background with GFP and tail-tip cells of a male BDF1 mouse as donor for nuclear transfer, respectively. Male 129B6F1 mice expressing GFP were generated by mating female 129/Sv mice with male C57BL/6 GFP transgenic mice (double-transgenic mouse line [16] constructed using pCAG-EGFP [10, 11] and Acr3-EGFP [17]).
この４行目にThe 129B6F1G1 [13] とあって13の注に2005年論文が指定されている。
そしてその後にSertoli cells of 129B6F1 background with GFPとあるね。そこまで読んでいるのに
次の文章にMale 129B6F1 mice expressing GFP were generated by mating female 129/Sv mice
with male C57BL/6 GFP transgenic mice (double-transgenic mouse line [16] constructed
using pCAG-EGFP [10, 11] and Acr3-EGFP [17]).と続いているのを読み過ごしている。
2005年論文に遡らなくても親の雌雄は再確認されているね。
2005年論文も多分流し読みされたわね。
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Mice
Immature male B6C3F1, adult female ICR, and adult female BDF1 mice were purchased from Shizuoka Laboratory Animal Center (Hamamatsu, Japan). Female 129/Sv-ter and male ICR n ude mice were purchased from CLEA Japan, Inc., and Charles River Japan, Inc., respectively. The green fluorescent protein (GFP) transgenic mice carrying both the acrosin/eGFP (Acr3-EGFP) [34] and pCX-eGFP [35] transgenes (C57BL/6TgN(acro/act-EGFP)OsbC3-N01-FJ002 [36]) were kindly provided by Dr. M. Okabe (Osaka University, Osaka, Japan; all strain designations are those of the original studies). To generate 129B6F1 mice carrying the GFP transgene, female 129/Sv-ter mice were mated with male C57BL/6 GFP transgenic mice, and the offspring of these matings, which were hemizygous for the GFP transgene, were used as donors for nuclear transplantation. All animal experiments conformed to the Guide for the Care and Use of Laboratory Animals and were approved by the Institutional Committee of Laboratory Animal Experimentation of the RIKEN Kobe Institute.
