忘却とは忘れ去ることなり。
Each slide was fixed with 4% paraformaldehyde (Wako) for 15 min at room temperature, washed with PBS, incubated with SuperBlock blocking buffer in PBS (P74370; Takara) for 30 min to block nonspecific reactions, and incubated with anti-c-kit rat monoclonal antibody (sc-19619; Santa Cruz Biotechnology, Inc), anti-Sca-1 rat monoclonal antibody (ab25031; Abcam), or anti-E-cadherin rat monoclonal antibody (ab11512; Abcam) overnight at 48C.
�B
専門的な手順を語ってるんでしょ。
After washing with PBS, the cells were incubated with goat anti-rat IgG Texas Red-conjugated antibodies (112-076-062;Jackson ImmunoResearch) and goat anti-rat IgG Fluorescein-conjugated antibodies (112-096-062; Jackson Immuno-Research) for 30 min at room temperature.
ここは彼女の専門ですからね。
Stage-specific embryonic antigen-1 and alkaline phosphatase staining was performed using the ES cell detection Kit (Millipore) in accordance with the manufacturer’s protocol.
少しは、興味が出てきましたか?それとも今日も釣りなの?
In negative controls, the primary antibody was replaced with IgG-negative controls of the same isotype to ensure specificity.
はいはい、詰まんないことやってないで前へ進んでください。
The spheres were collected at 5 days and dissociated into single cells, and placed in DMEM supplemented 20% fetal calf serum (FCS).
