It is generally felt that while ESCs and induced pluripotent stem cells demonstrate pluripotency, adult stems cells are limited to multipotency and are felt not to be able to cross the germ layer lineages as they develop.
and (adult stems cells) are felt not to be able to cross the germ layer lineages as they develop.
と続くんだから三胚葉分化以前の多能性を持っているものではないと信じられていると。
feelしているのは科学者じゃなくて我々ど素人たちという意味なのかなあ。私は三胚葉分化実験を
成功させたわよと言いたいんでしょ?
さて。
Although the existence of adult stem cells has been reported for more than a decade, we have hypothesized the existence of adult stem cells,which are quite small and resistant to external stress, that reside in tissues representative of the three germ layers.
3. Toma, J.G., Akhavan, M., Fernandes, K.J., Barnabe-Heider,F., Sadikot, A., Kaplan, D.R., and Miller, F.D. Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nat Cell Biol 3, 778, 2001.
4. Kucia, M., Reca, R., Campbell, F.R., Zuba-Surma, E., Majka, M., Ratajczak, J., and Ratajczak, M.Z.A population of very small embryonic-like (VSEL) CXCR4(þ)SSEA-1(þ)Oct-4þ stem cells identified in adult bone marrow. Leukemia 20, 857, 2006.
5.Jiang, Y., Jahagirdar, B.N., Reinhardt, R.L., Schwartz, R.E., Keene, C.D., Ortiz-Gonzalez, X.R., Reyes, M., Lenvik, T.,Lund, T., Blackstad, M., Du, J., Aldrich, S., Lisberg, A., Low, W.C., Largaespada, D.A., and Verfaillie, C.M. Pluripotency
of mesenchymal stem cells derived from adult marrow. Nature 418, 41, 2002.
だね。
we have hypothesized the existence of adult stem cells,which are quite
small and resistant to external stress, that reside in tissues representative
of the three germ layers.というのは、あくまで仮説としてこういうものを想定して
その実在の有無を確かめようとしているという意味だね。
Adult stem cells have generally been felt to be limited to multipotency and unable to cross germ layer lineages as they develop.って
アブストにもあった説明だね。feelという語彙選択はともかくとして、ここまでくると、こう感じでいる人々というのが、あげられた文献を
発表した人々だということらしいのは徐々に明らかになってきたかな。
続きだよ。
Cells derived from many adult tissues, including retina(6),brain(7-10),cornea(11),olfactory neuroepithelium(12、13), pancreas(14),skin(15,16),muscle(17),and bone marrow (BM)(18、19), have now been propagated as nonadherent clusters or spheres, as have ESCs(20).
この論旨の運びだとそうだよ。まだイントロなんだよ。彼女の知ってる
既存論文に書かれていることを知ってるぞと見せるところなんでしょうよ。
続きはこうだ。
In addition, neurospheres have been shown to contain stem cells demonstrating gene express that overlap that of ESCs.
808 ：ダウトファイア：2016/05/09(月) 06:47:03
＞Tissueに投稿しなおした博論の内容で書いた論文だけど、ES細胞がしっかり出てきちゃいますね。
FIG.2のデータでポジコンとしてＥＳ細胞を使っているね。マテメソにも。
"To eliminate the risk of false-positives,RNA was isolated from ES cells and E 6.5 fetal cells as positivecontrols, and RNA was also isolated from heart, muscle, li-ver, and adherent stromal cells as negative controls for em-bryonic gene marker expression in RT-PCR.
Cells derived from many adult tissues, including retina(6),brain(7-10),cornea(11),olfactory neuroepithelium(12、13), pancreas(14),skin(15,16),muscle(17),and bone marrow (BM)(18、19), have now been propagated as nonadherent clusters or spheres, as have ESCs(20).
いや、知識として知らないということは考えにくいんで、普段実際に自分の
実験で持ってこられた姿はnonadherent clusters or spheresではなかったかと。
無論、propagated as nonadherent clusters or spheresで、正しい記述になる
nonadherent の定義も考えられるんでしょ?
ま、その辺はね。後でいくらでも検討できる。進んでみましょうかね。
We believe that adult stem cells described in various reports represent the same adult stem cells at different stages of development, expressing different degrees of potency and that sphere-forming cells are much more immature than previously expected.
we have hypothesized the existence of adult stem cells,which are quite
small and resistant to external stress, that reside in tissues representative
of the three germ layers.
仮説構築はさらに続くぜ。
We hypothesized that adult stem cells procured from any tissue, ectoderm, mesoderm, or endoderm cross germ layers as they develop, when maintained in appropriate environments in vitro and in vivo.
テ、テ、テ。
In this study, we characterized cells isolated from three adult tissues (spinal cord, muscle, and lung) representative of the three different germ layers (ectoderm, mesoderm, and endoderm)and from BM.
