これ以外の情報はどこで無料で手に入れられるのか？
Modified STAP conditions facilitate bivalent fate decision between pluripotency and apoptosis in Jurkat T-lymphocytes
Jee Young Kim , Xinlai Cheng , Hamed Alborzinia , Stefan Wölfl,
Institute of Pharmacy and Molecular Biotechnology, Pharmaceutical Biology, Heidelberg University, Im Neuenheimer Feld 364, D-69120 Heidelberg, Germany
Received 8 February 2016, Accepted 8 March 2016, Available online 10 March 2016
Highlights
Sub-lethal acidity of Jurkat T-cells was newly defined with modified STAP protocol.
Onset of apoptosis (∼8 h) and plummeting cell viability (72 h) were identified.
T-lymphocytes' responses to acidic stress were irrelevant to OCT4A or OCT4B.
After the acidic stress, proportion of AP + and PI + populations increased.
Acidic stress didn't affect CSC or HSC maker expression in Jurkat cells.
Abstract
Low extracellular pH (pHe) is not only the result of cancer metabolism, but a factor of anti-cancer drug efficacy and cancer immunity. In this study, the consequences of acidic stress were evaluated by applying STAP protocol on Jurkat T-lymphocytes (2.0 × 106 cells/ml, 25 min in 37 °C). We detected apoptotic process exclusively in pH 3.3 treated cells within 8 h with western blotting (WB). This programmed cell death led to significant drop of cell viability in 72 h measured by MTT assay resulting PI positive population on flow cytometry (FCM) at day 7. Quantified RT-PCR (qRT-PCR) data indicated that all of above mentioned responses are irrelevant to expression of OCT4 gene variants. Interestingly enough, pluripotent cells represented by positive alkaline phosphatase (AP) staining survived acidic stress and consequently proportion of AP positive cells was significantly increased after pH 3.3 treatment (day 7). In general, acidic treatment led to an apoptotic condition for Jurkat T-lymphocytes, which occurred independent of OCT4 induction.
Thank you very much for your email-letters and your reminder today.
Because of important deadlines, I overlooked your first email request.
I did not expect to generate such an uproar especially with a small publication in
BBRC and must admit that I am quite overwhelmed with the attention that this small piece of work got.
We had selected this title because, we tried to repeat the STAP protocol immediately
after the Obokata paper came our, but with no success. With my PhD student we
wanted to understand a little bit better what may be the reason for the fact that
pH-stress also in our lab activated pluripotency markers but did never result in iPS cells.
The final outcome is the paper you mentioned and I hope that we were
very critical with putting our results in the right perspective.
If one reads our paper carefully it should be clear that we did not
obtain pluripotent cells that could be cultivated and propagated,
but rather observe some commonly used
markers using a modification of the "STAP protocol".
I hope that our results will be treated as they are and not used to
support unsubstantiated believes.
With best regards
stefan wölfl
Prof. Dr. Stefan Wölfl
Institut für Pharmazie und Molekulare Biotechnologie
Universität Heidelberg
Im Neuenheimer Feld 364
69120 Heidelberg
+49-6221-544878
+49-6221-544884 (fax)
wolfl@uni-hd.de
こんな英文書いてたら小保方先生に怒られるだろう。
彼女はreadmission to other universitiesと書いて内心の思いを読み取らずに
悪意で語彙選択の間違いを指摘するアホたちと戦ってるというのに。
こんな人達が学者だなんて、噓だわと思うに違いない。
実際うちの高一の息子でももう少しマシだ。
