1.The user must know what the site is about in seconds.
2.Make the content scannable.
3.Do not use fancy fonts that are unreadable.
4.Do not use tiny fonts.
5.Do not open new browser windows.
6.Do not resize the user’s browser windows.
7.Do not require a registration unless it is necessary.
8.Never subscribe the visitor for something without his consent.
9.Do not overuse Flash.
10.Do not play music.
11.If you MUST play an audio file let the user start it.
12.Do not clutter your website with badges.
13.Do not use a homepage that just launches the "real" website.
14.Make sure to include contact details.
15.Do not break the “Back” button.
16.Do not use blinking text.
17.Avoid complex URL structures.
18.Use CSS over HTML tables.
19.Make sure users can search the whole website.
20.Avoid "drop down" menus.
21.Use text navigation.
22.If you are linking to PDF files disclose it.
23.Do not confuse the visitor with many versions.
24.Do not blend advertising inside the content.
25.Use a simple navigation structure.
26.Avoid "intros".
27.Do not use FrontPage.
28.Make sure your website is cross-browser compatible.
29.Make sure to include anchor text on links.
30.Do not cloak links.
31.Make links visible.
32.Do not underline or color normal text.
33.Make clicked links change color.
34.Do not use animated GIFs.
35.Make sure to use the ALT and TITLE attributes for images.
36.Do not use harsh colors.
37.Do not use pop ups.
38.Avoid Javascript links.
39.Include functional links on your footer.
40.Avoid long pages.
41.No horizontal scrolling.
42.No spelling or grammatical mistakes.
43.If you use CAPTCHA make sure the letters are readable.
9.Do not overuse Flash.(フラッシュをこれみよがしに使うな)
10.Do not play music.(来訪者にいきなり音を発するな)
11.If you MUST play an audio file let the user start it.
(もし、音源や動画を使いたい場合は来訪者にスタートボタンを押させろ)