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>while a small number aggregates gave rise to colonies containing small cells with large nuclei, resembling the morphology of embryonic stem cells. However, most of these cells ceased proliferation at day 7 and gradually regressed. <Investigation of the cellular reprogramming phenomenon referred to as stimulus-triggered acquisition of pluripotency (STAP) Hitoshi Niwa>
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Notably, when cultured in LIF+FBS-containing medium for 4 days, Fgf4-induced stem cells underwent substantial changes in morphology and started to form ES-cell-like compact colonies with strong GFP signals (Fig. 3a). These cells showed expression of pluripotency makers, but not trophoblast markers (Fig. 3b and Extended Data Fig. 6a), and formed teratomas in mice (Extended Data Fig. 6b). These ES-like cells were generated from Fgf4-induced stem cells sorted for strong expression of the trophoblast marker Itga7, but rarely from Itga7-dim cells (Fig. 3c, d).
