ヤンさんの論文の小保方さんが言ってる部分は以下だわね。
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Results
Lif Increases the Efficiency of EpiSC Reprogramming
We compared the frequency of Epi-iPSC generation in the presence or absence of Lif. We used embryo-derived Oct4-GFP (O4G) reporter EpiSCs stably transfected with expression constructs for Klf4 or Nanog (Guo et al., 2009). On transfer from activin plus Fgf into 2i with Lif, these cells produced GFP-positive iPSC colonies at a frequency of 0.5%–1% (Guo et al., 2009, Silva et al., 2009). Without Lif or in the presence of a Jak inhibitor, this yield was reduced several fold (Figure 1A ). To test whether the effect of Lif is due simply to increased efficiency of iPSC self-renewal, we plated reprogrammed Epi-iPSCs in 2i with or without Lif. We observed no significant difference in numbers of colonies formed or their undifferentiated phenotype (Figure 1B).
<Without Lif or in the presence of a Jak inhibitor, this yield was
reduced several fold (Figure 1A ). >のところね。図の1-Aは棒グラフだけど
小保方さんがLetter Extendes Data5で示した写真と同じ結果ね。
もちろんだよ、ワトソン。
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However, as Fgf4-induced stem cells lay between STAP stem cells and trophoblast stem cells in the dendrogram, the possibility of contamination of STAP stem cells in the Fgf4-induced stem-cell population cannot be ruled out.
