やっと話がもとに巻き戻されてきわね。
Article Figure 2のf-iのリジェンドだわ。
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f–i, Dissociation culture of ES cells and STAP cells (additional 7 days from day 7; f, g) on gelatin-coated dishes. Top, bright-field; bottom, alkaline phosphatase (AP) staining. Partially dissociated STAP cells slowly generated small colonies (i), whereas dissociated STAP cells did not, even in the presence of the ROCK inhibitor (g, h), which allows dissociation culture of EpiSCs[29].
[29]は以下よ。
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29. Ohgushi, M. et al. Molecular pathway and cell state responsible for dissociation-induced apoptosis in human embryonic stem cells. Cell Stem Cell 7, 225–239 (2010)
Extended Data Figigure 5-d-hのリジェンドは以下よ。
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c, No substantial change in chromosome number was seen with STAP cells in the CGH array. Genomic DNA derived from CD45+ cells (male) was used as reference DNA. The spikes (for example, those seen in the X chromosome) were nonspecific and also found in the data of these parental CD45+ cells when the manufacturer’s control DNA was used as a reference. d, qPCR analysis for pluripotency markers that highly express in ES cells, but not in EpiSCs. Average ± s.d. e, Immunostaining of markers for mouse EpiSC and ES cells. Scale bar, 100 μm. f, g, H3K27me3+ foci in female cells, which are indicative of X-chromosomal inactivation. These foci were not observed in male cells. Scale bar, 10 μm. In the case of female STAP cells, ~40% of cells retained H3K27me3+ foci (g). **P < 0.001; Tukey’s test. n = 3, average ± s.d. Although nuclear staining looked to be higher in STAP cells with H3K27me3+ foci (f), this appeared to be caused by some optical artefacts scattering from the strong focal signal. h, qPCR analysis for the tight junction markers Zo-1 and claudin 7, which were highly expressed in EpiSCs, but not in ES cells or STAP cells. **P < 0.01; ns, not significant; Tukey's test; n = 3, average ± s.d.
